微阵列表达谱和生物信息学分析揭示膀胱癌中环状RNA的异常表达
Microarray Expression Profiles and Bioinformatics Analyses Reveal Aberrant Circular RNAs Expression in Bladder Cancer.
作者信息
Yang Jun, Chen Junwen, Wu Si, Fei Xiang, Wang Xia, Wang Kefeng
机构信息
Department of Gastroenterology, Shengjing Hospital of China Medical University, Shenyang 110004, People's Republic of China.
Department of Urology, Shengjing Hospital of China Medical University, Shenyang 110004, People's Republic of China.
出版信息
Onco Targets Ther. 2020 Oct 27;13:10889-10899. doi: 10.2147/OTT.S270747. eCollection 2020.
BACKGROUND
Increasing evidence shows that circular RNAs (circRNAs) are involved in many biological processes, functioning as microRNA (miRNA) sponges. The aim of this study is to identify differentially expressed circRNAs in bladder cancer (BCa).
METHODS
The transcriptome of circRNAs in BCa was assayed by microarray. Quantitative real-time PCR was performed to verify the results. Then, potential miRNA response elements (MREs) between circRNAs and miRNAs were predicted. Pathway and ontology enrichment analyses were performed to identify mechanisms related to the gene regulation of differentially expressed circRNAs.
RESULTS
Three hundred and eighty-six up-regulated and 394 down-regulated circRNAs were identified, and their potential MREs were predicted in BCa.
CONCLUSION
The differentially expressed circRNAs indicate that circRNAs could play important roles in the molecular pathogenesis of BCa.
背景
越来越多的证据表明，环状RNA（circRNA）参与许多生物学过程，充当微小RNA（miRNA）海绵。本研究的目的是鉴定膀胱癌（BCa）中差异表达的circRNA。
方法
通过微阵列分析BCa中circRNA的转录组。进行定量实时PCR以验证结果。然后，预测circRNA与miRNA之间的潜在微小RNA反应元件（MRE）。进行通路和本体富集分析以鉴定与差异表达circRNA的基因调控相关的机制。
结果
在BCa中鉴定出386个上调的circRNA和394个下调的circRNA，并预测了它们的潜在MRE。
结论
差异表达的circRNA表明circRNA可能在BCa的分子发病机制中发挥重要作用。
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