Evaluation of the genetic relatedness of isolates by TRs analysis.

OBJECTIVES

Human gastrointestinal tract harbors a variety of bacteria with vital roles in human health. is considered one of the dominant constituents of gut microflora which can act as an opportunistic pathogen leading to various diseases, including colon cancer, diarrhea, uterine and intrathecal abscesses, septicemia, and pelvic inflammation. In this study, multiple locus variable number of tandem repeats analysis (MLVA) was performed to genetically differentiate 50 isolates.

MATERIALS AND METHODS

Eight suitable tandem repeats (TRs) were selected by bioinformatics tools and were then subjected to PCR amplification using specific primers. Finally, MLVA profiles were clustered using BioNumerics 7.6 software package.

RESULTS

All VNTR loci were detected in all isolates using the PCR method. Overall, isolates were differentiated into 27 distinct MLVA types. The highest diversity index was allocated to TR1, TR2, TR5, TR6, and TR8; with this taken into account, strain type 14 was the most prevalent with 12 strains belonging to this type. Clustering revealed three major clusters of A, B, and C. With regards to the pathogenicity of and the outcomes of infections related to this microorganism, it is imperative to study this microorganism isolated from both patients and healthy individuals.

CONCLUSION

This study aimed at evaluating the efficiency of MLVA for the genetic differentiation of . The results of this study indicate the promising efficiency of MLVA typing for cluster detection of this bacterium.