Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Department of Physician Assistant Studies, School of Allied Health Sciences, University of Cape Coast, PMB, Cape Coast, Ghana.
Biomed Res Int. 2020 Oct 13;2020:9060356. doi: 10.1155/2020/9060356. eCollection 2020.
Deficient spermatozoon motility is one of the main causes of male infertility. However, there are still no accurate and effective treatments in a clinical setting for male asthenospermia. Exploring the genes and mechanism of asthenospermia has become one of the hot topics in reproductive medicine. Our aim is to study the effect of SLRIP on human spermatozoon motility and oxidative stress.
Sperm samples were collected including a normospermia group (60 cases) and an asthenospermia group (50 cases). SLIRP protein expression in spermatozoa was examined by western blotting, and relative mRNA expression of SLIRP in spermatozoa was quantified by reverse transcription polymerase chain reaction. Levels of reactive oxygen species (ROS), adenosine triphosphate (ATP) content, and the activity of manganese superoxide dismutase (MnSOD) in spermatozoa were also measured.
The mRNA level and protein expression of SLIRP in the asthenospermia group were significantly reduced compared with those in the normospermia group. The ROS active oxygen level in the asthenospermia group significantly increased; however, the ATP content decreased significantly as well as the activity of MnSOD.
SLIRP regulates human male fertility, and SLIRP and sperm progressive motility are positively correlated. The expression of SLIRP is declined, oxidative damage is increased, and energy metabolism is decreased in spermatozoa of asthenospermia patients compared to normospermia participants.
精子运动能力缺陷是男性不育的主要原因之一。然而，在临床实践中，对于男性弱精症仍然没有准确有效的治疗方法。探索弱精症的基因和机制已成为生殖医学的热点之一。我们的目的是研究 SLRIP 对人精子运动和氧化应激的影响。
收集精子样本，包括正常精子组（60 例）和弱精症组（50 例）。通过 Western blot 检测精子中 SLIRP 蛋白的表达，通过反转录聚合酶链反应定量检测精子中 SLIRP 的相对 mRNA 表达。还测量了精子中活性氧（ROS）、三磷酸腺苷（ATP）含量和锰超氧化物歧化酶（MnSOD）的活性。
与正常精子组相比，弱精症组的 SLIRP mRNA 水平和蛋白表达显著降低。弱精症组的 ROS 活性氧水平显著升高；然而，ATP 含量显著下降，MnSOD 的活性也显著降低。
SLIRP 调节男性生育能力，SLIRP 与精子前向运动呈正相关。与正常精子组相比，弱精症患者的精子中 SLIRP 表达下降，氧化损伤增加，能量代谢降低。
