Morabito F, Prasthofer E F, Dunlap N E, Grossi C E, Tilden A B
Department of Medicine, University of Alabama, Birmingham 35294.
Blood. 1987 Dec;70(6):1750-7.
We analyzed the expression of myelomonocytic-associated antigens on lymphocytes from B cell chronic lymphocytic leukemia (B-CLL) patients. Blood mononuclear cells were depleted of monocytes by one-step Percoll density gradient centrifugation and tested for antigen expression by fluorescent microscopy and flow cytometry. The reactivity of patient lymphocytes was as follows: 26 of 31 were positive for CD14 (Myr), 22 of 31 for a monocyte Fc receptor (MFC-1), 22 of 31 for CD11b (C3bi receptor), eight of 31 for CD15 (Leu-M1), five of 18 for CD13 (My 7), seven of 18 for My 9, and five of 30 for Mo 2. The B lymphocytes of B-CLL patients were also tested for the ability to produce interleukin 1 (IL-1) after depletion of monocytes and T lymphocytes. In 13 of 17 cases, B lymphocytes of patients produced IL-1 as detected in a mouse thymocyte proliferation assay and, in selected cases, a radioimmunoassay specific for IL-1 beta. The 13 cases that produced IL-1 were also positive for the expression of one or more myelomonocytic-associated antigens, whereas the four cases that did not produce IL-1 lacked expression of these antigens. In conclusion, the malignant B cells of B-CLL patients frequently express a variety of antigens generally considered specific for myelomonocytic cells, and expression of these antigens is associated with the ability to produce IL-1.
我们分析了B细胞慢性淋巴细胞白血病（B-CLL）患者淋巴细胞上髓单核细胞相关抗原的表达情况。通过一步法Percoll密度梯度离心去除血液单核细胞中的单核细胞，然后用荧光显微镜和流式细胞术检测抗原表达。患者淋巴细胞的反应性如下：31例中有26例CD14（Myr）呈阳性，31例中有22例单核细胞Fc受体（MFC-1）呈阳性，31例中有22例CD11b（C3bi受体）呈阳性，31例中有8例CD15（Leu-M1）呈阳性，18例中有5例CD13（My 7）呈阳性，18例中有7例My 9呈阳性，30例中有5例Mo 2呈阳性。在去除单核细胞和T淋巴细胞后，还检测了B-CLL患者B淋巴细胞产生白细胞介素1（IL-1）的能力。在17例中的13例中，患者的B淋巴细胞在小鼠胸腺细胞增殖试验中检测到产生了IL-1，在选定的病例中，还通过针对IL-1β的放射免疫测定法进行了检测。产生IL-1的13例患者中，一种或多种髓单核细胞相关抗原的表达也呈阳性，而未产生IL-1的4例患者则缺乏这些抗原的表达。总之，B-CLL患者的恶性B细胞经常表达多种通常被认为是髓单核细胞特异性的抗原，这些抗原的表达与产生IL-1的能力相关。
