Laver J, Jhanwar S C, O'Reilly R J, Castro-Malaspina H
Memorial Sloan-Kettering Cancer Center Laboratories of Developmental Hematopoiesis, New York, NY 10021.
Blood. 1987 Dec;70(6):1966-8.
The origin of marrow stromal cells post allogeneic bone marrow transplantation (BMT) was studied. Two groups of patients receiving HLA-identical marrow grafts from sex mismatched siblings were included in the study: the first group (eight patients) received conventional marrow grafts and the second group (ten patients) received stromal cell and T cell depleted grafts. All patients showed hematopoietic engraftment with donor cells. Marrow aspirates obtained from these patients were used to establish stromal layers in long-term marrow cultures (LTMC) for 4 to 6 weeks. In both groups, karyotype analysis of nonhematopoietic cultured stromal cells showed host origin even as late as day 760 posttransplantation. Immunofluorescence methods using monoclonal antibodies against components of fibroblasts, macrophages, and endothelial cells, showed that the composition of stromal layers was similar to those obtained from normal controls. Our data indicate that marrow stromal progenitors capable of proliferation are nontransplantable and do not originate from a hematopoietic-stromal common progenitor.
对异基因骨髓移植（BMT）后骨髓基质细胞的起源进行了研究。该研究纳入了两组接受来自性别不匹配同胞的 HLA 相同骨髓移植的患者：第一组（8 名患者）接受传统骨髓移植，第二组（10 名患者）接受去除基质细胞和 T 细胞的移植。所有患者均显示供体细胞的造血植入。从这些患者获取的骨髓抽吸物用于在长期骨髓培养（LTMC）中建立基质层 4 至 6 周。在两组中，非造血培养基质细胞的核型分析显示，即使在移植后 760 天，基质细胞仍来源于宿主。使用针对成纤维细胞、巨噬细胞和内皮细胞成分的单克隆抗体的免疫荧光方法表明，基质层的组成与从正常对照获得的相似。我们的数据表明，能够增殖的骨髓基质祖细胞不可移植，且并非源自造血 - 基质共同祖细胞。
