Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea.
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Gyeongnam, Republic of Korea.
J Vet Intern Med. 2020 Nov;34(6):2545-2554. doi: 10.1111/jvim.15940. Epub 2020 Nov 5.
High-mobility group box 1 (HMGB1) is a key mediator of neuroinflammation and there are increased HMGB1 levels in laboratory animal models of epilepsy and human patients with epilepsy.
To determine serum HMGB1 levels in dogs with epilepsy.
Twenty-eight epileptic dogs, 12 dogs with nonepileptic brain diseases, and 26 healthy dogs.
In this case-control study, serum HMGB1 concentrations were estimated using the canine-specific enzyme-linked immunosorbent assay kit. Diagnosis of dogs with epilepsy was based on medical history, physical and neurological examination findings, laboratory test results, magnetic resonance image, and cerebrospinal fluid analysis.
Serum HMGB1 levels were significantly higher in epileptic dogs (median = 0.41 ng/mL; range, 0.03-5.28) than in healthy dogs (median = 0.12 ng/mL; range, 0.02-1.45; P = .002). In contrast, serum HMGB1 levels of dogs with non-epileptic brain diseases (median = 0.19 ng/mL; range, 0.03-1.04) were not significantly increased compared to those of healthy dogs (P = .12). Regarding idiopathic epilepsy, dogs with an epilepsy course of >3 months showed a higher serum HMGB1 concentration (median = 0.87 ng/mL; range, 0.42-2.88) than those with that of ≤3 months (median = 0.26 ng/mL; range, 0.03-0.88; P = .02).
Serum HMGB1 could be a biomarker of epilepsy.
高迁移率族蛋白 B1（HMGB1）是神经炎症的关键介质，在癫痫的实验室动物模型和人类癫痫患者中，HMGB1 水平升高。
确定癫痫犬的血清 HMGB1 水平。
28 只癫痫犬、12 只非癫痫性脑部疾病犬和 26 只健康犬。
在这项病例对照研究中，使用犬特异性酶联免疫吸附测定试剂盒估计血清 HMGB1 浓度。癫痫犬的诊断基于病史、体格检查和神经学检查结果、实验室检查结果、磁共振成像和脑脊液分析。
癫痫犬的血清 HMGB1 水平明显高于健康犬（中位数=0.41ng/mL；范围，0.03-5.28；P=0.002）。相比之下，非癫痫性脑部疾病犬的血清 HMGB1 水平（中位数=0.19ng/mL；范围，0.03-1.04）与健康犬相比并未显著升高（P=0.12）。关于特发性癫痫，病程>3 个月的犬血清 HMGB1 浓度较高（中位数=0.87ng/mL；范围，0.42-2.88），病程≤3 个月的犬血清 HMGB1 浓度较低（中位数=0.26ng/mL；范围，0.03-0.88；P=0.02）。
血清 HMGB1 可能是癫痫的生物标志物。
