Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea.
College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.
J Vet Intern Med. 2024 Mar-Apr;38(2):1074-1082. doi: 10.1111/jvim.17008. Epub 2024 Feb 8.
High concentrations of complement factors are presented in serum of animal epilepsy models and human patients with epilepsy.
To determine whether complement dysregulation occurs in dogs with idiopathic epilepsy (IE).
The study included 49 dogs with IE subgrouped into treatment (n = 19), and nontreatment (n = 30), and 29 healthy dogs.
In this case-control study, the serum concentrations of the third (C3) and fourth (C4) components of the complement system were measured using a canine-specific ELISA kit.
Serum C3 and C4 concentrations were significantly higher in dogs with IE (C3, median; 4.901 [IQR; 3.915-6.673] mg/mL, P < .001; C4, 0.327 [0.134-0.557] mg/mL, P = .03) than in healthy control dogs (C3, 3.550 [3.075-4.191] mg/mL; C4, 0.267 [0.131-0.427] mg/mL). No significant differences were observed in serum C3 and C4 concentrations between dogs in the treatment (C3, median; 4.894 [IQR; 4.192-5.715] mg/mL; C4, 0.427 [0.143-0.586] mg/mL) and nontreatment groups (C3, 5.051 [3.702-7.132] mg/mL; C4, 0.258 [0.130-0.489] mg/mL). Dogs with a seizure frequency >3 times/month had significantly higher serum C3 (6.461 [4.695-8.735] mg/mL; P < .01) and C4 (0.451 [0.163-0.675] mg/mL; P = .01) concentrations than those with a seizure frequency ≤3 times/month (C3, 3.859 [3.464-5.142] mg/mL; C4, 0.161 [0.100-0.325] mg/mL).
Dysregulation of classical complement pathway was identified in IE dogs. Serum C3 and C4 concentrations could be diagnostic biomarkers for IE in dogs with higher seizure frequency.
在动物癫痫模型和癫痫患者的血清中存在高浓度的补体因子。
确定特发性癫痫（IE）犬是否存在补体失调。
本研究纳入了 49 只 IE 犬，分为治疗组（n=19）和非治疗组（n=30），以及 29 只健康犬。
在这项病例对照研究中，使用犬特异性 ELISA 试剂盒测定补体系统的第三（C3）和第四（C4）成分的血清浓度。
IE 犬的血清 C3 和 C4 浓度显著高于健康对照组（C3，中位数；4.901[IQR；3.915-6.673]mg/mL，P<0.001；C4，0.327[0.134-0.557]mg/mL，P=0.03）。治疗组（C3，中位数；4.894[IQR；4.192-5.715]mg/mL；C4，0.427[0.143-0.586]mg/mL）和非治疗组（C3，中位数；5.051[3.702-7.132]mg/mL；C4，0.258[0.130-0.489]mg/mL）之间的血清 C3 和 C4 浓度无显著差异。每月发作频率>3 次的犬的血清 C3（6.461[4.695-8.735]mg/mL；P<0.01）和 C4（0.451[0.163-0.675]mg/mL；P=0.01）浓度显著高于每月发作频率≤3 次的犬（C3，3.859[3.464-5.142]mg/mL；C4，0.161[0.100-0.325]mg/mL）。
IE 犬存在经典补体途径失调。血清 C3 和 C4 浓度可能是癫痫发作频率较高的 IE 犬的诊断生物标志物。
