Wang Na, Liu Haipeng, Ma Bingqian, Zhao Ting, Chen Yanan, Yang Yongguang, Zhao Pan, Han Xiong
Department of Neurology, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, Henan, 450003, China.
Department of Neurological Rehabilitation, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450003, China.
Epilepsy Res. 2021 Nov;177:106767. doi: 10.1016/j.eplepsyres.2021.106767. Epub 2021 Sep 15.
Extracellular high-mobility group box 1 (HMGB1) is considered a proinflammatory mediator and is involved in various neurological disorders. This study aims to determine the expression profiles of HMGB1 in cerebrospinal fluid (CSF) and paired serum, and to explore whether there is a relationship between CSF HMGB1 concentrations with seizure parameters in adult patients with epilepsy.
CSF and paired serum HMGB1 concentrations were measured in patients with drug-refractory epilepsy (DRE, n = 27), newly diagnosed epilepsy (NDE, n = 56), and other non-inflammatory neurological disorders (ONNDs, n = 22). The correlations in HMGB1 levels between CSF and blood were performed. The associations between HMGB1 levels and seizure parameters were analyzed.
Mean (± SD) CSF HMGB1 concentrations were 5.08 ± 3.06, 3.03 ± 2.25, 0.83 ± 0.77 ng/mL in patients with DRE, NDE, and ONNDs, respectively. Corresponding mean (± SD) serum concentrations were 4.53 ± 2.81, 2.32 ± 1.54, 1.56 ± 0.84 ng/mL. The CSF HMGB1 concentrations were significantly higher in the DRE and NDE groups compared with the ONNDs group (p < 0.001). There were no correlations in HMGB1 levels between CSF and serum in the DRE, NDE, and ONNDs groups. Furthermore, patients with symptomatic etiology showed significantly high levels of CSF HMGB1. Patients without remission expressed elevated levels of CSF HMGB1 at one-year follow-up. Additionally, the CSF HMGB1 levels were positively associated with seizure frequency.
Our study shows that HMGB1 may be a critical player in seizure mechanisms and CSF HMGB1 might be predictive in determining epilepsy etiology and prognosis.
细胞外高迁移率族蛋白B1（HMGB1）被认为是一种促炎介质，参与多种神经系统疾病。本研究旨在确定脑脊液（CSF）和配对血清中HMGB1的表达谱，并探讨成年癫痫患者脑脊液中HMGB1浓度与癫痫发作参数之间是否存在关系。
对药物难治性癫痫（DRE，n = 27）、新诊断癫痫（NDE，n = 56）和其他非炎性神经系统疾病（ONNDs，n = 22）患者测定脑脊液和配对血清中HMGB1浓度。分析脑脊液和血液中HMGB1水平的相关性。分析HMGB1水平与癫痫发作参数之间的关联。
DRE、NDE和ONNDs患者的脑脊液HMGB1平均（±标准差）浓度分别为5.08±3.06、3.03±2.25、0.83±0.77 ng/mL。相应的血清平均（±标准差）浓度为4.53±2.81、2.32±1.54、1.56±0.84 ng/mL。与ONNDs组相比，DRE组和NDE组的脑脊液HMGB1浓度显著更高（p < 0.001）。DRE、NDE和ONNDs组的脑脊液和血清中HMGB1水平无相关性。此外，有症状病因的患者脑脊液HMGB1水平显著升高。未缓解的患者在一年随访时脑脊液HMGB1水平升高。此外，脑脊液HMGB1水平与癫痫发作频率呈正相关。
我们的研究表明，HMGB1可能是癫痫发作机制中的关键因素，脑脊液HMGB1可能对确定癫痫病因和预后具有预测作用。
