榆林合剂对多囊卵巢综合征模型小鼠 miR-320/SF-1/Cyp19a1 的初步研究。
Preliminary study of Yulin mixture affecting the miR-320/SF-1/Cyp19a1 on mouse polycystic ovary syndrome model.
机构信息
Department of Pharmacy, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
出版信息
Gynecol Endocrinol. 2021 Jun;37(6):546-553. doi: 10.1080/09513590.2020.1843623. Epub 2020 Nov 5.
In this study, the polycystic ovary syndrome (PCOS) mice model was randomly divided into 6 groups: blank control group, Clomiphene group, PCOS group, and Yulin mixture high-/medium-/low-dose group. Rats were killed after 5 weeks of administration. The expression levels of serum E,T,Insulin and LH were detected by ELISA. The localizations and quantities of Steroid-generating factor-1 (SF-1) and Cytochrome protein P450 a1 (Cyp19a1) were detected by immunohistochemistry and western blot. The quantities of miR-320 were detected by RT-PCR. The results showed that the mechanism of Yulin mixture inhibiting the growth of polycystic ovary on mouse PCOS model may be through the decreasing of serum T and LH levels and then reducing local estrogen content to make the polycystic ovary atrophy. Yulin mixture can decrease the level of miR-320 and increase the expression of SF-1 and Cyp19a1 in ovary, thereby regulating the ovarian granulosa cell proliferation and apoptosis.
在这项研究中，多囊卵巢综合征（PCOS）小鼠模型被随机分为 6 组：空白对照组、氯米芬组、PCOS 组和玉林合剂高/中/低剂量组。给药 5 周后处死大鼠。通过 ELISA 检测血清 E、T、胰岛素和 LH 的表达水平。通过免疫组织化学和 Western blot 检测类固醇生成因子-1（SF-1）和细胞色素蛋白 P450a1（Cyp19a1）的定位和数量。通过 RT-PCR 检测 miR-320 的含量。结果表明，玉林合剂抑制 PCOS 模型小鼠多囊卵巢生长的机制可能是通过降低血清 T 和 LH 水平，进而降低局部雌激素含量，使多囊卵巢萎缩。玉林合剂可降低 miR-320 的水平，增加卵巢中 SF-1 和 Cyp19a1 的表达，从而调节卵巢颗粒细胞的增殖和凋亡。
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