Department of Pharmacy, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Gynecol Endocrinol. 2021 Jun;37(6):546-553. doi: 10.1080/09513590.2020.1843623. Epub 2020 Nov 5.
In this study, the polycystic ovary syndrome (PCOS) mice model was randomly divided into 6 groups: blank control group, Clomiphene group, PCOS group, and Yulin mixture high-/medium-/low-dose group. Rats were killed after 5 weeks of administration. The expression levels of serum E,T,Insulin and LH were detected by ELISA. The localizations and quantities of Steroid-generating factor-1 (SF-1) and Cytochrome protein P450 a1 (Cyp19a1) were detected by immunohistochemistry and western blot. The quantities of miR-320 were detected by RT-PCR. The results showed that the mechanism of Yulin mixture inhibiting the growth of polycystic ovary on mouse PCOS model may be through the decreasing of serum T and LH levels and then reducing local estrogen content to make the polycystic ovary atrophy. Yulin mixture can decrease the level of miR-320 and increase the expression of SF-1 and Cyp19a1 in ovary, thereby regulating the ovarian granulosa cell proliferation and apoptosis.
在这项研究中，多囊卵巢综合征（PCOS）小鼠模型被随机分为 6 组：空白对照组、氯米芬组、PCOS 组和玉林合剂高/中/低剂量组。给药 5 周后处死大鼠。通过 ELISA 检测血清 E、T、胰岛素和 LH 的表达水平。通过免疫组织化学和 Western blot 检测类固醇生成因子-1（SF-1）和细胞色素蛋白 P450a1（Cyp19a1）的定位和数量。通过 RT-PCR 检测 miR-320 的含量。结果表明，玉林合剂抑制 PCOS 模型小鼠多囊卵巢生长的机制可能是通过降低血清 T 和 LH 水平，进而降低局部雌激素含量，使多囊卵巢萎缩。玉林合剂可降低 miR-320 的水平，增加卵巢中 SF-1 和 Cyp19a1 的表达，从而调节卵巢颗粒细胞的增殖和凋亡。
