Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agriculture Science, Hangzhou, China.
Department of Reproduction, the Third of Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.
Reprod Domest Anim. 2021 Jan;56(1):112-119. doi: 10.1111/rda.13854. Epub 2020 Nov 17.
For successful batch farrowing, porcine oestrus and ovulation must be synchronized using fixed-time artificial insemination (FTAI). However, exogenous gonadotropins, which are currently used in FTAI, negatively affect gilt ovulation. Here, we aimed to improve sexually mature gilt superovulation efficiency using passive immunization against inhibin during FTAI. Altrenogest-treated gilts were challenged with 10 ml anti-inhibin serum (AIS group, n = 6), 1,000 IU pregnant mare serum gonadotropin (PMSG group, n = 6), or 10 ml goat serum (control group, n = 6). Gilts in the AIS and PMSG groups were inseminated according to the FTAI protocol, and gilts in the control group were inseminated during natural oestrus. When PMSG was replaced by AIS during FTAI of gilts, ovulation rate and embryos recovered were significantly greater in the AIS group as compared to the other two groups (p < .05). Especially the average number of 6-8-cell embryos in the AIS group was significantly higher than that in the PMSG group (p < .01). Moreover, the blastocyst number in the AIS group was significantly higher than that in the PMSG group and the control group (p < .05). But there was no significant difference in the blastocyst number between the PMSG group and the control group (p > .05). Besides, plasma levels of estradiol-β (E2) and progesterone (P4) were significantly greater in the AIS group as compared to the other two groups on Day 23 and D 27, respectively (p < .01). In summary, we devised an improved high-yield FTAI protocol for sexually mature gilts using AIS; this protocol had a greater superovulation efficiency than the FTAI using PMSG.
为了成功进行批次分娩，必须使用定时人工授精（FTAI）同步猪的发情和排卵。然而，目前用于 FTAI 的外源性促性腺激素会对后备母猪的排卵产生负面影响。在这里，我们旨在通过在 FTAI 期间使用抑制素的被动免疫来提高性成熟后备母猪的超排效率。用烯丙孕素处理的后备母猪分别接受 10ml 抗抑制素血清（AIS 组，n=6）、1000IU 孕马血清促性腺激素（PMSG 组，n=6）或 10ml 山羊血清（对照组，n=6）处理。AIS 和 PMSG 组的后备母猪按照 FTAI 方案进行授精，对照组的后备母猪在自然发情时进行授精。当 PMSG 在 FTAI 期间被 AIS 取代时，AIS 组的排卵率和回收胚胎数明显高于其他两组（p<0.05）。特别是 AIS 组的 6-8 细胞胚胎的平均数量明显高于 PMSG 组（p<0.01）。此外，AIS 组的囊胚数量明显高于 PMSG 组和对照组（p<0.05）。但 PMSG 组和对照组的囊胚数量之间没有显著差异（p>0.05）。此外，在第 23 天和第 27 天，AIS 组的雌二醇-β（E2）和孕酮（P4）的血浆水平明显高于其他两组（p<0.01）。综上所述，我们设计了一种使用 AIS 改进的成熟后备母猪高效 FTAI 方案；该方案的超排效率比使用 PMSG 的 FTAI 方案更高。
