Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou 450046, China.
Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou 450046, China.
Food Chem. 2021 Mar 1;339:128079. doi: 10.1016/j.foodchem.2020.128079. Epub 2020 Sep 13.
In this work, an efficient method for the determination of β-agonists and fluoroquinolones was established, based on a mixed-mode sorbent of magnetic sulfonated covalent organic framework composites. By coupling with HPLC-MS/MS, the main factors that affect the extraction procedure were optimized. Under the optimal conditions, the proposed HPLC-MS/MS method was successfully utilized for the extraction of β-agonists and fluoroquinolones in milk and pork meat samples. The method showed good linearities (R ≥ 0.9916), and low LOQs of 0.1-0.2 ng g for β-agonists and fluoroquinolones. The adsorption mechanism was investigated with the assistance of quantum chemistry calculation method, and it is worth noting that the sorbent relied mainly on the multiple adsorption mechanisms, including π-π stacking, hydrophobic, electrostatic attraction and hydrogen-bonding interactions. This work not only provides a simple method for the preparation of a mixed-mode sorbent, but also a routine analysis strategy for monitoring the illegal use of β-agonists and fluoroquinolones.
在这项工作中，建立了一种基于磁性磺化共价有机骨架复合材料的混合模式吸附剂来测定β-激动剂和氟喹诺酮类药物的高效方法。通过与 HPLC-MS/MS 联用，优化了影响萃取过程的主要因素。在最佳条件下，该 HPLC-MS/MS 方法成功地用于牛奶和猪肉样品中β-激动剂和氟喹诺酮类药物的提取。该方法表现出良好的线性（R≥0.9916），β-激动剂和氟喹诺酮类药物的低检出限为 0.1-0.2ng g。吸附机理通过量子化学计算方法进行了研究，值得注意的是，吸附剂主要依赖于多种吸附机制，包括π-π堆积、疏水、静电吸引和氢键相互作用。这项工作不仅提供了一种制备混合模式吸附剂的简单方法，而且还提供了一种常规的分析策略，用于监测β-激动剂和氟喹诺酮类药物的非法使用。
