Institute of Food & Nutrition Science and Technology, Shandong Academy of Agricultural Sciences, Jinan, China.
Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.
Mikrochim Acta. 2024 May 6;191(6):297. doi: 10.1007/s00604-024-06386-1.
A new detection platform based on a hydroxylated covalent organic framework (COF) integrated with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was constructed and used for detecting adrenergic receptor agonists (ARAs) residues in milk. The hydroxylated COF was prepared by polymerization of tris(4-aminophenyl)amine and 1,3,5-tris(4-formyl-3-hydroxyphenyl)benzene and applied to solid-phase extraction (SPE) of ARAs. This hydroxylated COF was featured with hierarchical flower-like morphology, easy preparation, and copious active adsorption sites. The adsorption model fittings and molecular simulation were applied to explore the potential adsorption mechanism. This detection platform was suitable for detecting four α- and five β-ARAs residues in milk. The linear ranges of the ARAs were from 0.25 to 50 µg·kg; the intra-day and the inter-day repeatability were in the range 2.9-7.9% and 2.0-10.1%, respectively. This work demonstrates this hydroxylated COF has great potential as SPE cartridge packing, and provides a new way to determine ARAs residues in milk.
构建了一种基于羟化共价有机框架（COF）与液相色谱-串联质谱（LC-MS/MS）集成的新型检测平台，用于检测牛奶中肾上腺素能受体激动剂（ARAs）残留。羟化 COF 通过三（4-氨基苯基）胺和 1,3,5-三（4-醛基-3-羟基苯基）苯的聚合制备，并应用于 ARAs 的固相萃取（SPE）。这种羟化 COF 具有分级花状形态、易于制备和丰富的活性吸附位点。采用吸附模型拟合和分子模拟来探究潜在的吸附机制。该检测平台适用于检测牛奶中四种 α-和五种 β-ARAs 残留。ARAs 的线性范围为 0.25-50 µg·kg；日内和日间重复性分别在 2.9-7.9%和 2.0-10.1%范围内。这项工作表明，这种羟化 COF 具有作为 SPE 柱填充剂的巨大潜力，并为测定牛奶中 ARAs 残留提供了一种新方法。
