Technische Universität Kaiserslautern, Department of Chemistry, Division of Food Chemistry and Toxicology, Erwin-Schrödinger-Str. 52, 67663 Kaiserslautern, Germany.
AB SCIEX Germany GmbH, 64293 Darmstadt, Germany.
Molecules. 2020 Nov 3;25(21):5104. doi: 10.3390/molecules25215104.
We have investigated urine samples after coffee consumption using targeted and untargeted approaches to identify furan and 2-methylfuran metabolites in urine samples by UPLC-qToF. The aim was to establish a fast, robust, and time-saving method involving ultra-performance liquid chromatography-quantitative time-of-flight tandem mass spectrometry (UPLC-qToF-MS/MS). The developed method detected previously reported metabolites, such as Lys-BDA, and others that had not been previously identified, or only detected in animal or in vitro studies. The developed UPLC-qToF method detected previously reported metabolites, such as lysine--2-butene-1,4-dial (Lys-BDA) adducts, and others that had not been previously identified, or only detected in animal and in vitro studies. In sum, the UPLC-qToF approach provides additional information that may be valuable in future human or animal intervention studies.
我们使用靶向和非靶向方法研究了咖啡消费后的尿液样本，通过超高效液相色谱-定量飞行时间串联质谱（UPLC-qToF-MS/MS）鉴定尿液样本中的呋喃和 2-甲基呋喃代谢物。目的是建立一种快速、稳健、省时的方法，涉及超高效液相色谱-定量飞行时间串联质谱（UPLC-qToF-MS/MS）。所开发的方法检测到了以前报道的代谢物，如 Lys-BDA 以及其他以前未被识别或仅在动物或体外研究中检测到的代谢物。所开发的 UPLC-qToF 方法检测到了以前报道的代谢物，如赖氨酸-2-丁烯-1,4-二醛（Lys-BDA）加合物，以及其他以前未被识别或仅在动物和体外研究中检测到的代谢物。总之，UPLC-qToF 方法提供了可能对未来人类或动物干预研究有价值的额外信息。
