Department of Microbiology, Government Theni Medical College, Theni; Department of Microbiology, Dr ALM PG IBMS, University of Madras, Chennai; Department of Microbiology, Virus Research and Diagnostic Laboratory, Government Theni Medical College, Theni, Tamil Nadu, India.
Department of Microbiology; Department of Microbiology, Virus Research and Diagnostic Laboratory, Government Theni Medical College, Theni, Tamil Nadu, India.
Indian J Med Microbiol. 2020 Jul-Dec;38(3 & 4):401-408. doi: 10.4103/ijmm.IJMM_20_89.
Dengue fever (DF) is caused by an arthropod-borne dengue virus (DENV), has four serotypes and several genotypes. Although having clinical and epidemiological significance, the information on the circulating serotypes/genotypes is scarce in India.
Blood specimens were collected from the patients suspected of DF and they are tested for DENV NS1 antigen and DENV IgM by ELISA. Antigen-positive samples were further serotyped by reverse transcriptase polymerase chain reaction. Representative samples from each serotype were sequenced to identify the genotypes.
All the four DENV serotypes were detected with the pre-dominance of DENV-1 (n = 49; 41.9%). Cases with multiple DENV serotype infections were also identified. Genotyping showed that DENV-1 belonging to genotype I, DENV-2 cosmopolitan (IV), DENV-3 genotype III and DENV-4 genotype I were active in the circulation during the outbreak in 2017.
Our study documents the molecular characteristics of DENV circulating in our geographical locality. The detection of heterologous DENV serotypes highlights the importance of regular molecular monitoring for the early recognition of any switch in pre-dominant serotype.
登革热（DF）是由节肢动物传播的登革病毒（DENV）引起的，有四个血清型和几个基因型。尽管具有临床和流行病学意义，但印度对流行的血清型/基因型的信息知之甚少。
采集疑似 DF 患者的血液标本，通过 ELISA 检测 DENV NS1 抗原和 DENV IgM。对阳性抗原样本进一步进行逆转录聚合酶链反应（RT-PCR）进行血清分型。对每个血清型的代表性样本进行测序以确定基因型。
所有四个 DENV 血清型均被检测到，其中 DENV-1 （n = 49; 41.9%）占优势。还发现了多种 DENV 血清型感染的病例。基因分型显示，2017 年流行期间，DENV-1 属于基因型 I，DENV-2 属于世界性（IV），DENV-3 属于基因型 III，DENV-4 属于基因型 I。
本研究记录了在我们地理区域流行的 DENV 的分子特征。异源 DENV 血清型的检测强调了定期进行分子监测的重要性，以便及早发现优势血清型的任何变化。
