Department of Urology, The Second Affiliated Hospital, University of South China; Department of Urology, The Third People's Hospital of Yongzhou City, Hunan, China.
Department of Urinary Surgery, Hospital of Xi'an, Xi'an China, China.
Indian J Pathol Microbiol. 2020 Oct-Dec;63(4):575-580. doi: 10.4103/IJPM.IJPM_612_19.
This study aimed to investigate the expression of long non-coding RNA (lncRNA) small nucleolar RNA host gene 1 (SNHG1) in prostate cancer (PCa) patients and to assess the effects of SNHG1 on PCa cell proliferation and apoptosis.
A total of 134 PCa patients were randomly included from patients who underwent surgical resection at our hospital from October 2015 to December 2016. The SNHG1 expression levels in PCa tissues and paired adjacent non-cancerous tissues were detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The association of the SNHG1 expression with clinical-pathological features of PCa patients was summarized and evaluated. A short interfering (si) RNA targeting SNHG1 and pcDNA3.1-SNHG1 were transfected into PC3 and DU145 PCa cell lines, and transfection efficiency was verified by qRT-PCR. Cell proliferation and apoptosis were assessed by methylthiazolyldiphenyl-tetrazolium bromide (MTT) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays, respectively.
The SNHG1 expression was significantly upregulated in PCa tumor tissues compared with paired adjacent non-cancerous tissues. The SNHG1 expression was obviously associated with the TNM stage, Gleason Score, lymph node invasion, and long-term metastasis mortality rate. Silencing of SNHG1 inhibited cell proliferation and promoted apoptosis in PC3 and DU145 PCa cell lines in vitro, while overexpression of SNHG1 led to opposite results.
LncRNA SNHG1 was upregulated and associated with aggressive malignant behavior in PCa progression. SNHG1 might serve as a potential prognostic biomarker and potential therapeutic target for PCa.
本研究旨在探讨长链非编码 RNA（lncRNA）小核仁 RNA 宿主基因 1（SNHG1）在前列腺癌（PCa）患者中的表达，并评估 SNHG1 对 PCa 细胞增殖和凋亡的影响。
从 2015 年 10 月至 2016 年 12 月在我院接受手术切除的患者中随机纳入 134 例 PCa 患者。采用实时定量逆转录聚合酶链反应（qRT-PCR）检测 PCa 组织和配对的癌旁正常组织中 SNHG1 的表达水平。总结并评估 SNHG1 表达与 PCa 患者临床病理特征的关系。将靶向 SNHG1 的短发夹 RNA（siRNA）和 pcDNA3.1-SNHG1 转染至 PC3 和 DU145 PCa 细胞系中，通过 qRT-PCR 验证转染效率。采用甲基噻唑基四唑溴盐（MTT）和末端脱氧核苷酸转移酶 dUTP 缺口末端标记（TUNEL）法分别评估细胞增殖和凋亡。
与配对的癌旁正常组织相比，PCa 肿瘤组织中 SNHG1 的表达明显上调。SNHG1 的表达与 TNM 分期、Gleason 评分、淋巴结浸润和长期转移死亡率明显相关。体外沉默 SNHG1 抑制 PC3 和 DU145 PCa 细胞系的细胞增殖并促进细胞凋亡，而过表达 SNHG1 则导致相反的结果。
lncRNA SNHG1 在 PCa 进展中上调，并与侵袭性恶性行为相关。SNHG1 可能作为 PCa 的潜在预后生物标志物和潜在治疗靶点。
