Department of Urology Surgery, Shandong Provincial Third Hospital, No.12, Wuyingshan Middle Road, Tianqiao District, Jinan, Shandong, 250031, China.
Department of Joint Surgery, Shandong Provincial Third Hospital, Jinan, Shandong, 250031, China.
BMC Urol. 2024 Jul 12;24(1):144. doi: 10.1186/s12894-024-01531-7.
Prostate cancer, characterized by its insidious onset and short overall survival, and has seen a rise in incidence over recent decades. This study aims to investigate the expression and molecular mechanism of lncRNA PTCSC3 (PTCSC3) in prostate cancer in order to develop new prognostic and therapeutic biomarkers.
The level of PTCSC3 in serum and cell samples of prostate cancer was quantitatively measured using RT-qPCR assays. The correlation between the variation in PTCSC3 levels and clinical indicators of patients was evaluated. The survival status of the prostate cancer patients included in the study was evaluated using Kaplan-Meier curve and multivariable Cox analysis. The impact of PTCSC3 overexpression on cell growth and activity was revealed by CCK-8 and Transwell assays. The targeting relationship between PTCSC3 and miR-182-5p was determined by bioinformatics prediction and luciferase activity.
PTCSC3 was found to be downregulated in prostate cancer, and its low levels were associated with short overall survival in patients. It influenced the progression of prostate cancer by targeting miR-182-5p. Increasing PTCSC3 levels suppressed the proliferation, migration and invasion levels of cells, and miR-182-5p mimic counteracted PTCSC3's effects on cells.
As a potential prognostic biological factor for prostate cancer, PTCSC3 may regulate the progression of prostate cancer by sponging miR-182-5p and affect the prognosis of patients.
前列腺癌具有隐匿性发病和整体生存期短的特点，近年来发病率有所上升。本研究旨在探讨 lncRNA PTCSC3（PTCSC3）在前列腺癌中的表达及分子机制，以期为前列腺癌的预后和治疗提供新的生物标志物。
采用 RT-qPCR 检测前列腺癌患者血清和细胞样本中 PTCSC3 的水平，评估 PTCSC3 水平变化与患者临床指标的相关性。采用 Kaplan-Meier 曲线和多因素 Cox 分析评估研究中纳入的前列腺癌患者的生存状况。通过 CCK-8 和 Transwell 实验揭示 PTCSC3 过表达对细胞生长和活性的影响。通过生物信息学预测和荧光素酶活性测定确定 PTCSC3 与 miR-182-5p 的靶向关系。
PTCSC3 在前列腺癌中表达下调，其低水平与患者总体生存时间短有关。它通过靶向 miR-182-5p 影响前列腺癌的进展。增加 PTCSC3 水平可抑制细胞的增殖、迁移和侵袭水平，而 miR-182-5p 模拟物可拮抗 PTCSC3 对细胞的作用。
PTCSC3 作为前列腺癌潜在的预后生物学因素，可能通过海绵吸附 miR-182-5p 调节前列腺癌的进展，影响患者的预后。
