Department of GI Medicine, Rizhao People's Hospital, Rizhao, China.
Eur Rev Med Pharmacol Sci. 2020 Oct;24(20):10445-10451. doi: 10.26355/eurrev_202010_23396.
The aim of this study was to explore the expression level of Wnt1-inducible signaling pathway protein 1 (WISP1) and its clinical significance in hepatocellular carcinoma (HCC).
Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was performed to detect the expression level of WISP1 in HCC tissues and cells. Kaplan-Meier curves and Cox proportional hazard regression model were chosen for single and multiple factor analysis of survival analysis, respectively. Furthermore, wound healing assay and transwell assay were used to verify the effect of WISP1 on HCC cell metastasis in vitro.
The expression level of WISP1 in HCC tissues was significantly higher than that in para-cancer tissues (p<0.05). WISP1 expression was positively correlated with lymph node metastasis and clinical stage of HCC. Kaplan-Meier curve showed that HCC patients with higher WISP1 expression exhibited significantly worse progression free survival (PFS) time and overall survival (OS) time. Both univariate and multivariate analysis indicated that high expression of WISP1 was an independent predictor of poor prognosis in HCC. In addition, WISP1 significantly promoted the invasion and migration of HCC cells in vitro.
WISP1 might contribute to the development of HCC, serving as a clinical biomarker and therapeutic target for HCC patients.
本研究旨在探讨 Wnt1 诱导信号通路蛋白 1（WISP1）在肝细胞癌（HCC）中的表达水平及其临床意义。
采用实时荧光定量聚合酶链反应（qRT-PCR）检测 HCC 组织和细胞中 WISP1 的表达水平。采用 Kaplan-Meier 曲线和 Cox 比例风险回归模型分别进行单因素和多因素生存分析。此外，还进行了划痕愈合实验和 Transwell 实验，以验证 WISP1 对 HCC 细胞体外转移的影响。
WISP1 在 HCC 组织中的表达水平明显高于癌旁组织（p<0.05）。WISP1 的表达与 HCC 的淋巴结转移和临床分期呈正相关。Kaplan-Meier 曲线显示，WISP1 高表达的 HCC 患者的无进展生存期（PFS）和总生存期（OS）明显缩短。单因素和多因素分析均表明，WISP1 高表达是 HCC 患者预后不良的独立预测因素。此外，WISP1 显著促进了 HCC 细胞的侵袭和迁移。
WISP1 可能促进 HCC 的发展，有望成为 HCC 患者的临床生物标志物和治疗靶点。
