Department of Animal Production, Faculty of Agriculture at Moshtohor, Benha University, Qalyubia, Egypt.
Avian Pathology Section, Department of Veterinary Medicine, University of Bari, Bari, Italy.
Br Poult Sci. 2021 Apr;62(2):180-187. doi: 10.1080/00071668.2020.1847252. Epub 2020 Dec 14.
1.Four gallinacin () genes were assessed by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) to characterise these genes in Fayoumi (F) and Rhode Island Red (R) breeds and their crosses of Rhode Island Red Fayoumi (½R½F) and Fayoumi Rhode Island Red (½F½R).2.Genes examined were and . The molecular associations between the SNPs of the gallinacin genes and body weight, caecal bacterial count and the serum antibody titres of IgA, IgG and IgM were determined. In the R breed, the frequency of TC genotype was higher than TT and CC genotypes for the gene. The GG genotype frequency was higher than AA and AG genotypes for the gene in the other genetic groups, and the CA genotype frequency was higher than CC and AA genotypes in crosses for the gene.3.In all populations, the frequency of the C allele was higher than the T allele for the gene, the G allele was higher than the A allele for the gene and the C allele was higher than the A allele for the gene. The observed heterozygosity in R, ½R½F and ½F½R was 0.476, 0.375 and 0.158 for the gene, 0.458, 0.615 and 0.250 for the gene and 0.053, 0.792 and 0.739 for the gene, while the expected heterozygosities were 0.490, 0.430 and 0.145 for the gene, 0.430, 0.348 and 0.219 for the gene and 0.229, 0.478 and 0.496 for the gene, respectively.4.On a molecular level, the genotype TT was significantly higher for body weight than TC and CC genotypes in the gene. Birds with the GG genotype had a significantly lower count than birds with AA genotype in the gene. Birds with the genotype AA had higher significant body weights than those with CC and CA genotypes in the 5 gene.5.The results indicated that the and genes are potential candidates for selection programmes to improve resistance and body weight in chickens.
通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）评估了 4 种卵白蛋白基因，以表征法尤姆鸡（F）和罗得岛红鸡（R）及其罗得岛红鸡×法尤姆鸡（1/2R1/2F）和法尤姆鸡×罗得岛红鸡（1/2F1/2R）杂交鸡中的这些基因。
检测的基因是 和 。确定了卵白蛋白基因 SNP 与体重、盲肠细菌计数以及血清 IgA、IgG 和 IgM 抗体滴度之间的分子关联。在 R 品种中， 基因的 TC 基因型频率高于 TT 和 CC 基因型。在其他遗传群体中， 基因的 GG 基因型频率高于 AA 和 AG 基因型，而 基因的 CA 基因型频率在杂交中高于 CC 和 AA 基因型。
在所有群体中， 基因的 C 等位基因频率高于 T 等位基因频率， 基因的 G 等位基因频率高于 A 等位基因频率， 基因的 C 等位基因频率高于 A 等位基因频率。R、1/2R1/2F 和 1/2F1/2R 中的观测杂合度分别为 基因的 0.476、0.375 和 0.158， 基因的 0.458、0.615 和 0.250， 和 基因的 0.053、0.792 和 0.739，而预期杂合度分别为 基因的 0.490、0.430 和 0.145， 基因的 0.430、0.348 和 0.219， 和 基因的 0.229、0.478 和 0.496。
在分子水平上， 基因的 TT 基因型的体重显著高于 TC 和 CC 基因型。 基因的 GG 基因型的盲肠细菌计数显著低于 AA 基因型。 基因的 AA 基因型的体重显著高于 CC 和 CA 基因型。
结果表明， 和 基因是提高鸡对疾病的抵抗力和体重的选择计划的潜在候选基因。
