State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China.
Center of Laboratory Animal Science, Nanchang University, Nanchang, China.
J Sci Food Agric. 2021 May;101(7):2940-2949. doi: 10.1002/jsfa.10926. Epub 2020 Nov 28.
Tropomyosin (TM) is the major allergen of crustaceans. The allergenicity of TM from Macrobrachium nipponense (MnTM) and the anaphylactic reaction in the digestive tract are still unclear. The aim of this study was to evaluate the allergenicity of MnTM and the anaphylactic reaction in the digestive tract.
Serum IgE and IgG1 binding ability in the TM group were significantly higher than those in the PBS and CT groups (P < 0.01) and CP group (P < 0.05), while serum IgG and IgG2a binding ability showed no obvious difference between the four groups (P > 0.05). The levels of cytokines IL-4, IL-5 and IL-13 in TM and CP groups were significantly higher than those in PBS and CT groups. Histamine and β-hexosaminidase in the TM and CP groups from basophil cell models were significantly higher than those in the PBS group. The highest mRNA expression of IL-4 and IL-13 was in the jejunum from TM-sensitized mice. Histopathological analysis showed that more immune cells infiltrated into the jejunum than the duodenum and ileum from the TM-sensitized mice.
MnTM could promote an allergic response in mice and lead to degranulation in basophil cells. The jejunum was more easily affected by MnTM than duodenum and ileum, and the jejunum may be the major site of allergic response in the digestive tract. © 2020 Society of Chemical Industry.
原肌球蛋白（TM）是甲壳类动物的主要过敏原。MnTM 的变应原性和消化道过敏反应尚不清楚。本研究旨在评估 MnTM 的变应原性和消化道过敏反应。
TM 组血清 IgE 和 IgG1 结合能力明显高于 PBS 和 CT 组（P<0.01）和 CP 组（P<0.05），而 IgG 和 IgG2a 结合能力四组之间无明显差异（P>0.05）。TM 和 CP 组细胞因子 IL-4、IL-5 和 IL-13 的水平明显高于 PBS 和 CT 组。TM 和 CP 组嗜碱性粒细胞模型中的组胺和β-己糖胺酶明显高于 PBS 组。TM 致敏小鼠空肠中 IL-4 和 IL-13 的 mRNA 表达最高。组织病理学分析表明，与十二指肠和回肠相比，TM 致敏小鼠空肠中有更多的免疫细胞浸润。
MnTM 可促进小鼠发生过敏反应，并导致嗜碱性粒细胞脱颗粒。空肠比十二指肠和回肠更容易受到 MnTM 的影响，空肠可能是消化道过敏反应的主要部位。© 2020 英国化学学会。
