Plant Developmental Physiology and Genetics Laboratory, Department of Plant Science, Federal University of Santa Catarina, SC, 88034-001, Florianópolis, Brazil.
Institut de Systématique, Evolution, Biodiversité (ISYEB), Muséum National D'Histoire Naturelle, CNRS, Sorbonne Université, EPHE, 39, 57 rue Cuvier, F-750055, Paris, CP, France.
Mycorrhiza. 2021 Jan;31(1):1-15. doi: 10.1007/s00572-020-01000-7. Epub 2020 Nov 6.
The Serendipitaceae family was erected in 2016 to accommodate the Sebacinales 'group B' clade, which contains peculiar species of cultivable root-associated fungi involved in symbiotic associations with a wide range of plant species. Here we report the isolation of a new Serendipita species which was obtained from protocorms of the terrestrial orchid Epidendrum fulgens cultivated in a greenhouse. This species is described based on phylogenetic analysis and on its microscopic and ultrastructural features in pure culture and in association with the host's protocorms. Its genome size was estimated using flow cytometry, and its capacity to promote the germination of E. fulgens seeds and to associate with roots of Arabidopsis thaliana was also investigated. Serendipita restingae sp. nov. is closely related to Serendipita sp. MAFF305841, isolated from Microtis rara (Orchidaceae), from which it differs by 14.2% in the ITS region and by 6.5% in the LSU region. It produces microsclerotia formed of non-monilioid hyphae, a feature that was not reported for the Sebacinales hitherto. Serendipita restingae promoted the germination of E. fulgens seeds, forming typical mycorrhizal pelotons within protocorm cells. It was also able to colonize the roots of Arabidopsis thaliana under in vitro conditions. Arabidopsis plants grown in association with S. restingae increased their biomass more than fourfold. Serendipita restingae is the first Serendipitaceae species described for the Americas.
被囊菌科于 2016 年设立，以容纳 Sebacinales 'B 群' 分支，该分支包含与广泛植物物种共生的奇特栽培根相关真菌。在这里，我们报告了一种新的被囊菌物种的分离，该物种是从温室中栽培的陆生兰花兜兰的原球茎中获得的。该物种是基于系统发育分析以及在纯培养和与宿主原球茎共生时的微观和超微结构特征来描述的。使用流式细胞术估计了其基因组大小，并研究了其促进兜兰种子萌发和与拟南芥根共生的能力。Serendipita restingae sp. nov. 与从杓兰（Orchidaceae）中分离的 Serendipita sp. MAFF305841 密切相关，ITS 区相差 14.2%，LSU 区相差 6.5%。它产生由非 monilioid 菌丝形成的微菌核，这是 Sebacinales 迄今尚未报道的特征。Serendipita restingae 促进兜兰种子的萌发，在原球茎细胞内形成典型的菌根丛。它还能够在体外条件下定殖拟南芥的根。与 S. restingae 共生的拟南芥植物的生物量增加了四倍多。Serendipita restingae 是第一个被描述为来自美洲的被囊菌科物种。
