Nogueira Pedro T S, Freitas Emiliane F S, Silva Jessica A R, Kasuya Maria C M, Pereira Olinto L
Departamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
Departamento de Microbiologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
Braz J Microbiol. 2025 Mar;56(1):589-599. doi: 10.1007/s42770-024-01597-3. Epub 2024 Dec 27.
Orchidaceae is one of the largest plant families and stands out for its wide variety of flowers with ornamental and environmental importance. Cattleya is one of the main commercial genera, presenting a great diversity of species and hybrids that attract the attention of collectors, orchid enthusiasts, and consumers. In their natural environment, orchids associate with mycorrhizal fungi, which are responsible for providing carbon and other nutrients during seed germination. This study investigated the potential of mycorrhizal fungi isolated from the genus Cattleya for in vitro symbiotic germination of seeds from three contrasting Cattleya species, comparing them with non-symbiotic germination in a commercially used culture medium for orchid propagation. The isolated fungi were molecularly identified through phylogenetic analyses of DNA sequences using the ITS (Internal Transcribed Spacer) region. Three isolates obtained were identified as Tulasnella amonilioides, and through microscopic evaluations, the formation of monilioid cells was observed, a morphological characteristic previously unknown for this species. The T. amonilioides isolates were efficient in promoting seed germination of Cattleya bicolor, Cattleya walkeriana and Cattleya jongheana and accelerated the germination process when compared with the non-symbiotic commercial medium, showing to be promised for commercial seed production of these orchids species.
兰科是最大的植物科之一，以其种类繁多、具有观赏和环境重要性的花卉而闻名。卡特兰属是主要的商业属之一，拥有大量的物种和杂交品种，吸引了收藏家、兰花爱好者和消费者的关注。在自然环境中，兰花与菌根真菌共生，这些真菌在种子萌发过程中负责提供碳和其他营养物质。本研究调查了从卡特兰属分离出的菌根真菌对三种不同卡特兰属物种种子进行体外共生萌发的潜力，并将其与在商业上用于兰花繁殖的培养基中的非共生萌发进行比较。通过使用ITS（内部转录间隔区）区域对DNA序列进行系统发育分析，对分离出的真菌进行了分子鉴定。获得的三个分离株被鉴定为阿蒙利拟胶膜菌，通过显微镜评估，观察到了念珠状细胞的形成，这是该物种以前未知的形态特征。与非共生商业培养基相比，阿蒙利拟胶膜菌分离株在促进双色卡特兰、沃氏卡特兰和琼氏卡特兰种子萌发方面效率很高，并加速了萌发过程，显示出有望用于这些兰花物种的商业种子生产。
