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TOP1α、UPF1 和 TTG2 以亲本剂量依赖的方式调节种子大小。

TOP1α, UPF1, and TTG2 regulate seed size in a parental dosage-dependent manner.

机构信息

Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore.

Temasek Life Sciences Laboratory, National University of Singapore, Singapore.

出版信息

PLoS Biol. 2020 Nov 6;18(11):e3000930. doi: 10.1371/journal.pbio.3000930. eCollection 2020 Nov.

DOI:10.1371/journal.pbio.3000930
PMID:33156841
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7673560/
Abstract

Cues of maternal and paternal origins interact to control seed development, and the underlying molecular mechanisms are still far from clear. Here, we show that TOPOISOMERASE Iα (TOP1α), UP-FRAMESHIFT SUPPRESSOR 1 (UPF1), and TRANSPARENT TESTA GLABRA2 (TTG2) gametophytically, biparentally regulate seed size in Arabidopsis. TOP1α and UPF1 are mainly expressed in antipodal cells, and loss of their function leads to ectopic TTG2 expression in these female gametophytic cells. We further demonstrate that TOP1α and UPF1 directly repress TTG2 expression through affecting its chromatin status and determine its relative expression in antipodal cells versus sperm cells, which controls seed size in a dosage-dependent and parent-of-origin-dependent manner. The molecular interplay among these three genes explains their biparental gametophytic effect during diploidy and interploidy reciprocal crosses. Taken together, our findings reveal a molecular framework of parental interaction for seed size control.

摘要

亲本来源的线索相互作用以控制种子发育，而潜在的分子机制仍远未清楚。在这里，我们表明 TOPOISOMERASE Iα (TOP1α)、UP-FRAMESHIFT SUPPRESSOR 1 (UPF1) 和 TRANSPARENT TESTA GLABRA2 (TTG2) 在配子体中、双亲中调控拟南芥种子大小。TOP1α 和 UPF1 主要在对生细胞中表达，其功能丧失导致这些雌性配子体细胞中 TTG2 的异位表达。我们进一步证明 TOP1α 和 UPF1 通过影响其染色质状态直接抑制 TTG2 的表达，并确定其在对生细胞与精子细胞中的相对表达，以剂量依赖和亲本来源依赖的方式控制种子大小。这三个基因之间的分子相互作用解释了它们在二倍体和异倍体回交中的双亲配子体效应。总之，我们的研究结果揭示了一个控制种子大小的亲本相互作用的分子框架。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5f8/7673560/e63cf693cb76/pbio.3000930.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5f8/7673560/132d0e510f9c/pbio.3000930.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5f8/7673560/a35b76ab8543/pbio.3000930.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5f8/7673560/dc6a4433d743/pbio.3000930.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5f8/7673560/9a99a25432ee/pbio.3000930.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5f8/7673560/e1ebcb828ed6/pbio.3000930.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5f8/7673560/aec5f115df6e/pbio.3000930.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5f8/7673560/e63cf693cb76/pbio.3000930.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5f8/7673560/132d0e510f9c/pbio.3000930.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5f8/7673560/a35b76ab8543/pbio.3000930.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5f8/7673560/dc6a4433d743/pbio.3000930.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5f8/7673560/9a99a25432ee/pbio.3000930.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5f8/7673560/e1ebcb828ed6/pbio.3000930.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5f8/7673560/aec5f115df6e/pbio.3000930.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f5f8/7673560/e63cf693cb76/pbio.3000930.g007.jpg

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