College of Engineering, China Agricultural University, Box 191, Beijing 100083, China.
College of Engineering, China Agricultural University, Box 191, Beijing 100083, China.
Bioresour Technol. 2021 Jan;320(Pt A):124306. doi: 10.1016/j.biortech.2020.124306. Epub 2020 Oct 26.
Lignocellulose fractionation is a primary treatment to enhance cellulose accessibility and multi-component use. Herein, the development of a one-step fractionation is reported for cellulose enrichment from corn stover using a low concentration of peracetic acid combined with maleic acid (PAM). The effects of pretreatment parameters on the contents of cellulose, hemicellulose, and lignin were investigated. After cooking for 1 h at 130 °C with 1.5 wt% peracetic acid and 3 wt% maleic acid, 86.83% of corn stover cellulose remained in the solid residue while 88.21% of hemicellulose and 87.77% of lignin dissolved into the aqueous liquid. Hemicellulose was primarily hydrolyzed into xylose with 84.58% recovered during the PAM process. The cellulose-rich residue was enzymatically hydrolyzed with a glucose yield of 89.65%, which was two to three times that of untreated substrate. Generally, the proposed process offers a promising approach for efficient fractionation of lignocellulose under mild and environmental-friendly conditions.
木质纤维素分级是一种主要的处理方法，可提高纤维素的可及性和多组分利用。本文报道了一种使用低浓度过氧乙酸与马来酸（PAM）一步法从玉米秸秆中富集纤维素的方法。考察了预处理参数对纤维素、半纤维素和木质素含量的影响。在 130°C 下用 1.5wt%过氧乙酸和 3wt%马来酸蒸煮 1 小时后，86.83%的玉米秸秆纤维素残留在固体残渣中，而 88.21%的半纤维素和 87.77%的木质素溶解在水溶液中。在 PAM 过程中，半纤维素主要水解为木糖，回收 84.58%。纤维素丰富的残渣用酶水解，葡萄糖得率为 89.65%，是未处理底物的两到三倍。总的来说，该方法在温和、环保的条件下为木质纤维素的有效分级提供了一种有前景的方法。
