渗滤液微生物组图谱揭示了固体废弃物分解过程中细菌、古菌和真核生物的动态变化以及产甲烷功能。
Leachate microbiome profile reveals bacteria, archaea and eukaryote dynamics and methanogenic function during solid waste decomposition.
机构信息
Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China.
Environmental Microbiology and Ecology Research Center, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, Chongqing 400714, China; School of Resources and Environmental Engineering, Anhui University, Anhui 230039, China.
出版信息
Bioresour Technol. 2021 Jan;320(Pt A):124359. doi: 10.1016/j.biortech.2020.124359. Epub 2020 Nov 2.
Bacterial, archaeal, and eukaryotic community composition and dynamics in leachate during solid waste decomposition were investigated using Illumina MiSeq sequencing. The functional enzyme-encoding genes of methanogenic pathways were also predicted via PICRUSt. Succession of bacterial, archaeal, and eukaryotic community composition in aerobic phase (AP), anaerobic acid phase (ACP), and methanogenic phase (MP) was observed. The main representatives of microbial phyla, genera, and species significantly (p < 0.05) differed at least two phases. Protist Ciliophora occurred at ACP and was prevalent in MP, suggesting a short food chain establishment in the methanogenesis. Bacterial, archaeal, fungi and eukaryotic community structure were all pH and biochemical oxygen demand (BOD) dependent patter. Acetoclastic and hydrogenotrophic methanogenesis pathways with associated functional genes differed during solid waste decomposition and were inhibited in ACP.
采用 Illumina MiSeq 测序研究了固体废物分解过程中渗滤液中细菌、古菌和真核生物群落组成和动态。还通过 PICRUSt 预测了产甲烷途径的功能酶编码基因。在好氧相 (AP)、厌氧酸相 (ACP) 和产甲烷相 (MP) 中观察到细菌、古菌和真核生物群落组成的演替。微生物门、属和种的主要代表至少在两个相中显著不同 (p < 0.05)。原生动物纤毛虫发生在 ACP 中，在 MP 中流行，表明在甲烷生成中建立了短食物链。细菌、古菌、真菌和真核生物群落结构均与 pH 和生化需氧量 (BOD) 有关。与相关功能基因的乙酰化和氢营养型产甲烷途径在固体废物分解过程中不同，并在 ACP 中受到抑制。
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