Abrahamson I K, Ferguson I A, Rush R A
Centre for Neuroscience, Flinders University of South Australia, Bedford Park.
Dev Biol. 1987 Dec;124(2):551-6. doi: 10.1016/0012-1606(87)90508-2.
We report the presence of endogenous nerve growth factor (NGF) in chicken peripheral nerve. The molecule has been detected with antibodies to mouse salivary gland NGF, using immunohistochemical and immunoelectrophoretic techniques. Previous studies have shown that these antibodies inhibit the survival activity of extracts of chicken peripheral nerve. The NGF accumulated distal, but not proximal, to a ligature placed on a peripheral sympathetic nerve demonstrating that it was retrogradely transported. This transport was detected in intact nerve fibers as well as in nerves from which the peripheral target had been ablated 6 hr or 7 days previously. The results indicate that avian NGF is present in adult chicken peripheral nerves and that this molecule shares antigenic determinants with the mouse molecule. The results further demonstrate that regenerating neurons retrogradely transport NGF supplied by cells within the peripheral nerve (presumably Schwann). The possibility that these cells also provide NGF to intact neurons is discussed.
我们报告了鸡外周神经中内源性神经生长因子（NGF）的存在。利用免疫组织化学和免疫电泳技术，用针对小鼠唾液腺NGF的抗体检测到了该分子。先前的研究表明，这些抗体可抑制鸡外周神经提取物的存活活性。在置于外周交感神经上的结扎线远端而非近端积累了NGF，这表明它是逆向运输的。在完整的神经纤维以及外周靶标在6小时或7天前已被切除的神经中都检测到了这种运输。结果表明，成年鸡外周神经中存在禽类NGF，并且该分子与小鼠分子具有共同的抗原决定簇。结果进一步证明，再生神经元逆向运输外周神经内细胞（可能是施万细胞）提供的NGF。还讨论了这些细胞是否也为完整神经元提供NGF的可能性。
