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[链球菌的表面特征:评估技术]

[Surface characteristics of Streptococci: evaluation technics].

作者信息

Savoia D, Venesio T, Martinotti M G

机构信息

Istituto di Microbiologia, Università di Torino.

出版信息

G Batteriol Virol Immunol. 1986 Jan-Jun;79(1-6):61-76.

PMID:3315803
Abstract

We examined the surface characteristics of 41 strains of Streptococci by using the salt aggregation technique (SAT). While group B, C, D and G Streptococci showed a clearly hydrophilic surface, 57.5% of group A strains revealed so hydrophobic characters as to be autoaggregating. Either the hydrophobicity or the autoaggregation of these Streptococci decreased after subsequent culture passages. A comparison has been done for 5 strains by the hydrophobic interaction chromatography (HIC) demonstrating a good correlation between the two techniques. As the only presence of M protein did not seem to condition the surface characteristics, however treatments with different proteolytic enzymes modify greatly the bacterial surface suggesting the involvement of various protein structures.

摘要

我们使用盐凝集技术(SAT)检测了41株链球菌的表面特征。B组、C组、D组和G组链球菌显示出明显亲水的表面,而57.5%的A组菌株表现出疏水性特征以至于能够自动聚集。这些链球菌传代培养后,其疏水性或自动聚集性均降低。通过疏水相互作用色谱法(HIC)对5株菌株进行了比较,结果表明这两种技术之间具有良好的相关性。由于仅M蛋白的存在似乎并不决定表面特征,然而,用不同的蛋白水解酶处理会极大地改变细菌表面,这表明各种蛋白质结构参与其中。

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