Janicka Grazyna, Mikucka Agnieszka, Sekowska Alicja, Zwierzchlewski Tomasz, Wróblewski Marcin
Department of Microbiology and Nosocomial Infections, Medical University in Bydgoszcz, Poland.
Acta Microbiol Pol. 2002;51(1):23-30.
The hydrophobicity of the bacterial cell surface was evaluated via the salt aggregation test (SAT) in 58 strains (19 from the lower and 39 from the upper respiratory tracts) of Moraxella catarrhalis in hospitalized patients aged 25 to 65. Based on the SAT results, the strains were divided into three groups: autoaggregating (highly hydrophobic), hydrophobic, and hydrophilic. At a temperature of 37 degrees C, the autoaggregating, hydrophobic or hydrophilic properties did not depend on the choice of a medium, whereas at 22 degrees C the investigated properties did (p<0.0001). Taking into account the origin of the strains (lower vs. upper respiratory tract), it was found that: in the strains cultivated in liquid medium, both highly hydrophobic, hydrophobic and hydrophilic surfaces were present with a comparative frequency, independent of the strain isolation site and cultivation conditions; strains with highly hydrophobic and hydrophobic surfaces, but only those cultivated on solid media at 22 degrees C, were much more often isolated from sputum rather than from nose and throat swabs, whereas a statistically significant incidence of hydrophilic strains was found in samples from the upper rather than lower respiratory tract.
通过盐聚集试验（SAT）对25至65岁住院患者的58株卡他莫拉菌（19株来自下呼吸道，39株来自上呼吸道）的细菌细胞表面疏水性进行了评估。根据SAT结果，这些菌株被分为三组：自聚集（高度疏水）、疏水和亲水。在37摄氏度时，自聚集、疏水或亲水特性不取决于培养基的选择，而在22摄氏度时，所研究的特性则取决于培养基的选择（p<0.0001）。考虑到菌株的来源（下呼吸道与上呼吸道），发现：在液体培养基中培养的菌株中，高度疏水、疏水和亲水表面出现的频率相对比较均衡，与菌株分离部位和培养条件无关；具有高度疏水和疏水表面的菌株，但只有那些在22摄氏度的固体培养基上培养的菌株，更常从痰液中分离出来，而不是从鼻拭子和咽拭子中分离出来，而上呼吸道样本中亲水菌株的发生率在统计学上显著高于下呼吸道样本。
