Yamaguchi T
Department of Obstetrics and Gynecology, Hokkaido University School of Medicine, Sapporo, Japan.
Hokkaido Igaku Zasshi. 1987 Jul;62(4):537-43.
For investigating the relationship between maternal age and incidence of molar pregnancy, attempt was made to experiment in animal model. C57BL/6J strain mice were used for experimental animal model because the transition of reproductive age of these animals have a strong resemblance to those of human. It is proved that hydatidiform mole arises from fertilization of "an empty egg" resulting from either enucleation or inactivation of the female pronucleus. Therefore incidence of anuclear oocyte during in vitro maturation process was examined of oocytes obtained from aged mice's ovaries(403-503 days old) in comparison with those of young mice's(108-274d.o.). The results were as follows: 1) Timing of oocyte maturation and ooclasmic maturation rate were the same in aged mice as in mature (young) ones. 2) But incidence of abnormal oocytes such as having two polar bodies were significantly higher in aged than in young mice(9.2% versus 3.6%). (p less than 0.025) 3) Especially incidence of abnormal anuclear oocytes were also very high in aged mice(aged: young = 12.5%:3.6%). (p less than 0.05) This result suggest the increase of molar pregnancy during climacteric in human is due to increase of anuclear oocytes for oocyte maturation.
为了研究孕妇年龄与葡萄胎发病率之间的关系，尝试在动物模型上进行实验。选用C57BL/6J品系小鼠作为实验动物模型，因为这些动物的生殖年龄转变与人类非常相似。已证实，葡萄胎源于去核或雌原核失活所产生的“空卵”受精。因此，检测了从老年小鼠（403 - 503日龄）卵巢获取的卵母细胞在体外成熟过程中无核卵母细胞的发生率，并与年轻小鼠（108 - 274日龄）的进行比较。结果如下：1）老年小鼠的卵母细胞成熟时间和卵胞质成熟率与成熟（年轻）小鼠相同。2）但是，老年小鼠中出现两个极体等异常卵母细胞的发生率显著高于年轻小鼠（9.2%对3.6%）。（p小于0.025）3）特别是老年小鼠中异常无核卵母细胞的发生率也非常高（老年：年轻 = 12.5%：3.6%）。（p小于0.05）这一结果表明，人类更年期期间葡萄胎发病率的增加是由于卵母细胞成熟过程中无核卵母细胞数量的增加。
