Department of Hepatobiliary Surgery, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, Zhejiang, 325000, China; Marshall Laboratory of Biomedical Engineering, International Cancer Center, Laboratory of Evolutionary Theranostics (LET), School of Biomedical Engineering, Shenzhen University Health Science Center, Shenzhen, 518060, China.
Marshall Laboratory of Biomedical Engineering, International Cancer Center, Laboratory of Evolutionary Theranostics (LET), School of Biomedical Engineering, Shenzhen University Health Science Center, Shenzhen, 518060, China.
Biomaterials. 2021 Jan;267:120454. doi: 10.1016/j.biomaterials.2020.120454. Epub 2020 Oct 16.
The development of tumor acidic microenvironment-responsive theranostic agents is a research hotspot. Herein, we developed highly photostable amphiphilic croconium dye-anchored red blood cell membrane vesicle (denoted as LET-5) for tumor pH-responsive near-infrared fluorescence (NIRF) and photoacoustic (PA) duplex imaging-guided photothermal therapy. In tumor acidic microenvironment, both NIRF and PA signals of LET-5 were significantly enhanced and the photothermal effect of LET-5 was activated. Notably, cell membrane-based vesicle with enhanced stability and long blood circulation significantly improved the tumor accumulation of croconium dye, thus achieving better therapeutic effect than free croconium dye. These findings provide a promising approach to construct amphiphilic dye-anchored cell membrane vesicle for cancer theranostics.
肿瘤酸性微环境响应治疗剂的开发是一个研究热点。在此，我们开发了一种高稳定的双亲性克罗酮染料锚定的红细胞膜囊泡（表示为 LET-5），用于肿瘤 pH 响应近红外荧光（NIRF）和光声（PA）双模式成像引导光热治疗。在肿瘤酸性微环境中，LET-5 的 NIRF 和 PA 信号均显著增强，并且 LET-5 的光热效应被激活。值得注意的是，基于细胞膜的囊泡具有增强的稳定性和长循环时间，显著提高了克罗酮染料在肿瘤中的积累，从而实现了比游离克罗酮染料更好的治疗效果。这些发现为构建用于癌症治疗的双亲性染料锚定细胞膜囊泡提供了一种有前途的方法。
