Sophisticated Analytical Instrument Facility, CSIR-Central Drug Research Institute, Lucknow, 226031, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh - 201002, India.
Sophisticated Analytical Instrument Facility, CSIR-Central Drug Research Institute, Lucknow, 226031, India.
J Pharm Biomed Anal. 2021 Jan 30;193:113707. doi: 10.1016/j.jpba.2020.113707. Epub 2020 Oct 20.
Ultra Performance Liquid Chromatography coupled with hybrid triple quadrupole linear ion trap tandem mass spectrometry (UPLC-ESI-QqQ-MS/MS) method in multiple reaction monitoring (MRM) acquisition mode was developed and validated for identification and simultaneous determination of potential anti-diabetic and anti-malarial compounds in ethanolic extracts of different Artemisia species. The chromatographic separation was carried out on an Acquity BEH™ C column (1.7 μm, 2.1 × 50 mm) with 0.1 % (v/v) formic acid in water and acetonitrile as mobile phase under gradient condition in 6 min. The developed method was validated in terms of linearity, LOD, LOQ, precision, stability and recovery according to international conference on harmonization guidelines. The correlation coefficients of all the calibration curves were ≥0.9902 and recoveries ranged from 98.22 to 104.49% (RSD ≤2.18 %). Relative standard deviations of intra-day, inter-day precisions and stability were ≤ 1.04, 1.09 and 2.80 %, respectively. The quantitative results showed remarkable differences in the content of all the compounds in different Artemisia species. The quantitative values of each peak were summarized as mean ± SD. The statistical analysis for comparison of observed quantitative differences of each compound was done to show that they are statistically significant. In-vitro assessment of extracts of selected Artemisia species inhibited adipocyte differentiation in 3T3-L1 cells, hence it may have certain phytochemicals which are responsible for reducing obesity and related metabolic disorders.
建立了一种采用超高效液相色谱-电喷雾串联三重四极杆线性离子阱质谱联用技术（UPLC-ESI-QqQ-MS/MS）在多反应监测（MRM）采集模式下，对不同青蒿属植物乙醇提取物中的潜在抗糖尿病和抗疟化合物进行鉴定和同时定量分析的方法。色谱分离在 Acquity BEH™ C 柱（1.7 μm，2.1×50mm）上进行，采用 0.1%（v/v）甲酸水和乙腈作为流动相，在 6min 内采用梯度洗脱条件。该方法按照国际协调会议指南进行了线性、LOD、LOQ、精密度、稳定性和回收率验证。所有校准曲线的相关系数均≥0.9902，回收率范围为 98.22%至 104.49%（RSD≤2.18%）。日内、日间精密度和稳定性的相对标准偏差分别≤1.04%、1.09%和 2.80%。定量结果表明，不同青蒿属植物中所有化合物的含量存在显著差异。每个峰的定量值均总结为平均值±SD。对各化合物观察到的定量差异进行比较的统计分析表明，它们具有统计学意义。对选定青蒿属植物提取物在 3T3-L1 细胞中抑制脂肪细胞分化的体外评估表明，其可能含有某些具有减肥和相关代谢紊乱作用的植物化学物质。
