Trifan Adriana, Zengin Gokhan, Sinan Kouadio Ibrahime, Sieniawska Elwira, Sawicki Rafal, Maciejewska-Turska Magdalena, Skalikca-Woźniak Krystyna, Luca Simon Vlad
Department of Pharmacognosy, Grigore T. Popa University of Medicine and Pharmacy Iasi, 700115 Iasi, Romania.
Physiology and Biochemistry Research Laboratory, Department of Biology, Science Faculty, Selcuk University, University Campus, 42130 Konya, Turkey.
Antioxidants (Basel). 2022 May 21;11(5):1017. doi: 10.3390/antiox11051017.
The L. genus comprises over 500 species with important medicinal and economic attributes. Our study aimed at providing a comprehensive metabolite profiling and bioactivity assessment of five species collected from northeastern Romania ( L., L., Jacq., L. and L.). Liquid chromatography-tandem high-resolution mass spectrometry (LC-HRMS/MS) analysis of methanol and chloroform extracts obtained from the roots and aerial parts of the plants led to the identification of 15 phenolic acids (mostly hydroxycinnamic acid derivatives), 26 flavonoids (poly-hydroxylated/poly-methoxylated flavone derivatives, present only in the aerial parts), 14 sesquiterpene lactones, 3 coumarins, 1 lignan and 7 fatty acids. Clustered image map (CIM) analysis of the phytochemical profiles revealed that was similar to and that was similar to whereas represented a cluster of its own. Correlated with their total phenolic contents, the methanol extracts from both parts of the plants showed the highest antioxidant effects, as assessed by the DPPH and ABTS radical scavenging, CUPRAC, FRAP and total antioxidant capacity methods. extracts proved to be promising sources of enzyme inhibitory agents, with the methanol aerial part extracts being the most active samples against acetylcholinesterase and glucosidase. All samples displayed good antibacterial effects against H37Ra, with MIC values of 64-256 mg/L. In conclusion, the investigated species proved to be rich sources of bioactives endowed with antioxidant, enzyme inhibitory and anti-mycobacterial properties.
L.属包含500多种具有重要药用和经济价值的物种。我们的研究旨在对从罗马尼亚东北部采集的五个物种（L.、L.、Jacq.、L.和L.）进行全面的代谢物谱分析和生物活性评估。对从植物根和地上部分获得的甲醇和氯仿提取物进行液相色谱 - 串联高分辨率质谱（LC - HRMS/MS）分析，鉴定出15种酚酸（主要是羟基肉桂酸衍生物）、26种黄酮类化合物（多羟基/多甲氧基黄酮衍生物，仅存在于地上部分）、14种倍半萜内酯、3种香豆素、1种木脂素和7种脂肪酸。植物化学图谱的聚类图像映射（CIM）分析表明，与相似，与相似，而自成一类。与它们的总酚含量相关，通过DPPH和ABTS自由基清除、CUPRAC、FRAP和总抗氧化能力方法评估，植物两部分的甲醇提取物显示出最高的抗氧化作用。提取物被证明是有前景的酶抑制剂来源，甲醇地上部分提取物是对乙酰胆碱酯酶和葡萄糖苷酶最具活性的样品。所有样品对H37Ra均显示出良好的抗菌效果，MIC值为64 - 256 mg/L。总之，所研究的物种被证明是具有抗氧化、酶抑制和抗分枝杆菌特性的生物活性物质的丰富来源。
