Laboratory of Cellular Toxicology, Faculty of Sciences, Department of Biology, University of Badji Mokhtar, Annaba, B.P. 12, Annaba, 23000, Algeria.
Laboratory of Cellular Toxicology, Faculty of Sciences, Department of Biology, University of Badji Mokhtar, Annaba, B.P. 12, Annaba, 23000, Algeria.
Chemosphere. 2021 Apr;268:128808. doi: 10.1016/j.chemosphere.2020.128808. Epub 2020 Oct 28.
Thiram (TMTD) is able to induce antioxidant defense and oxidative stress in different organisms. Moreover, Thiram can act as a prooxidant resulting in the formation of reactive oxygen species (ROS). To our knowledge, this is the first study assessing the oxidative stress induced by Thiram in the cladoceran Daphnia magna. At present, literature focus on the determination of toxicity in vertebrate organisms or cells, however, very few studies were interested to evaluate Thiram's effects in aquatic organisms such as cladoceran. To assess these effects, antioxidant GSH content, CAT and GST enzyme activities, cellular damages and lipid peroxidation indicators (MDA) were evaluated as oxidative stress biomarkers. Our results showed that acute Thiram exposure resulted in significant biochemical responses, demonstrating that Thiram induced oxidative damage. Indeed, following exposure to Thiram, we noticed an intracellular (GSH) depletion, associated with a marked increase of lipid membrane peroxidation as shown by high (MDA) production. Moreover, a dose-dependent induction of antioxidant key enzymes (CAT) and (GST) was found which led to an oxidative stress and finally death of Daphnia magna.
代森锰锌能够诱导不同生物体的抗氧化防御和氧化应激。此外，代森锰锌可以作为一种促氧化剂，导致活性氧（ROS）的形成。据我们所知，这是首次评估代森锰锌对枝角类动物大型溞（Daphnia magna）诱导的氧化应激的研究。目前，文献主要集中在确定脊椎动物或细胞的毒性上，然而，很少有研究有兴趣评估代森锰锌对水生生物如枝角类动物的影响。为了评估这些影响，我们将抗氧化剂 GSH 含量、CAT 和 GST 酶活性、细胞损伤和脂质过氧化指标（MDA）作为氧化应激生物标志物进行了评估。我们的结果表明，急性代森锰锌暴露会导致明显的生化反应，表明代森锰锌诱导了氧化损伤。事实上，在接触代森锰锌后，我们注意到细胞内（GSH）耗竭，同时脂质膜过氧化作用明显增加，表现为（MDA）产量高。此外，还发现抗氧化关键酶（CAT）和（GST）的剂量依赖性诱导，导致氧化应激，最终导致大型溞死亡。
