Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 60-965 Poznan, Poland.
University of Silesia in Katowice, Faculty of Natural Science, Institute of Biology, Biotechnology and Environmental Protection, Jagiellońska 28, 40 032 Katowice, Poland.
J Hazard Mater. 2021 Apr 5;407:124352. doi: 10.1016/j.jhazmat.2020.124352. Epub 2020 Oct 24.
Antibiotic residues in the environment may negatively affect biological communities in the natural ecosystems. However, their influence on environmental bacterial strains has not been thoroughly investigated. In this study, two representatives of 5-nitrofuran antibiotics (nitrofurantoin and furaltadone) were investigated in terms of their long-term influence on the cell envelopes of newly isolated environmental bacterial strains (Sphingobacterium caeni FTD2, Achromobacter xylosoxidans NFZ2 and Pseudomonas hibiscicola FZD2). A 12-month exposure of bacterial cells to nitrofurans at a concentration of 20 mg L induced changes in the cell structure and texture (bacteria under stress conditions showed a loss of their original shape and seemed to be vastly inflated, the cells increased average surface roughness after exposure to NFT and FTD, respectively). AFM observations allowed the calculation of the bacterial cell nanomechanical properties. Significant increase in adhesion energy of bacteria after prolonged contact with nitrofurantoin was demonstrated. Changes in the permeability of bacterial membrane, fatty acids' composition and bacterial cell surface hydrophobicity were determined. Despite visible bacterial adaptation to nitrofurans, prolonged presence of pharmaceuticals in the environment has led to significant alterations in the cells' structures which was particularly visible in P. hibiscicola.
环境中的抗生素残留可能会对自然生态系统中的生物群落产生负面影响。然而，它们对环境中细菌菌株的影响尚未得到彻底研究。在这项研究中，我们研究了两种 5-硝基呋喃类抗生素（呋喃妥因和呋喃他酮）对新分离的环境细菌菌株（鞘氨醇单胞菌 FTD2、木糖氧化无色杆菌 NFZ2 和 Hibiscicola hibiscicola FZD2）的细胞膜的长期影响。将细菌细胞在 20mg/L 的浓度下暴露于硝基呋喃类抗生素 12 个月，会引起细胞结构和质地的变化（在应激条件下的细菌失去了原来的形状，似乎被极度膨胀，暴露于 NFT 和 FTD 后，细胞的平均表面粗糙度分别增加）。原子力显微镜观察允许计算细菌细胞的纳米力学特性。证明了细菌在与硝基呋喃类抗生素长时间接触后，粘附能显著增加。还确定了细菌膜的通透性、脂肪酸组成和细菌细胞表面疏水性的变化。尽管细菌对硝基呋喃类抗生素有明显的适应性，但这些药物在环境中的长期存在导致了细胞结构的显著改变，在 Hibiscicola hibiscicola 中尤为明显。
