Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China.
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China; Comparative Endocrinology and Integrative Biology, Centre of Marine Sciences, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.
Comp Biochem Physiol B Biochem Mol Biol. 2021 Feb-Mar;252:110534. doi: 10.1016/j.cbpb.2020.110534. Epub 2020 Nov 5.
Dopamine beta-hydroxylase (DβH) plays an essential role in the synthesis of catecholamines (CA) in neuroendocrine networks. In the razor clam, Sinonovacula constricta a novel gene for DβH (ScDβH-α) was identified that belongs to the copper type II ascorbate-dependent monooxygenase family. Expression analysis revealed ScDβH-α gene transcripts were abundant in the liver and expressed throughout development. Knock-down of ScDβH-α in adult clams using siRNA caused a reduction in the growth rate compared to control clams. Reduced growth was associated with strong down-regulation of gene transcripts for the growth-related factors, platelet derived growth factors A (PDGF-A) (P < 0.001) 24 h after ScDβH-α knock-down, vascular endothelial growth factor (VEGF1) (P < 0.001) and platelet derived growth factor B (PDGF-B-2) (P < 0.001) 24 h and 48 h after ScDβH-α knock-down and transforming growth factor beta (TGF-β1) (P < 0.001) 48 h and 72 h after ScDβH-α knock-down. Taken together the results suggest that the novel ScDβH-α gene through its role in CA synthesis is involved in growth regulation in the razor clam and possibly other bivalves.
多巴胺β-羟化酶（DβH）在神经内分泌网络中儿茶酚胺（CA）的合成中发挥着重要作用。在刀额新对虾（Sinonovacula constricta）中，鉴定出一种新的 DβH 基因（ScDβH-α），属于铜 II 型抗坏血酸依赖性单加氧酶家族。表达分析显示 ScDβH-α基因转录本在肝脏中丰富，并在整个发育过程中表达。用 siRNA 敲低成年蛤蜊中的 ScDβH-α会导致与对照蛤蜊相比生长速度降低。生长速度降低与生长相关因子血小板衍生生长因子 A（PDGF-A）（P < 0.001）、血管内皮生长因子（VEGF1）（P < 0.001）和血小板衍生生长因子 B（PDGF-B-2）（P < 0.001）的基因转录物强烈下调有关，敲低后 24 小时和 48 小时，转化生长因子β（TGF-β1）（P < 0.001）敲低后 48 小时和 72 小时。综上所述，结果表明，新型 ScDβH-α基因通过其在 CA 合成中的作用，参与了刀额新对虾的生长调节，可能也参与了其他双壳类动物的生长调节。
