多巴胺 β-羟化酶及其在调控缢蛏生长和幼虫变态中的作用。
Dopamine beta-hydroxylase and its role in regulating the growth and larval metamorphosis in Sinonovacula constricta.
机构信息
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources and College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources and College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China.
出版信息
Gene. 2020 May 5;737:144418. doi: 10.1016/j.gene.2020.144418. Epub 2020 Feb 14.
Dopamine beta-hydroxylase (DβH) plays a key role in the synthesis of catecholamines (CAs) in the neuroendocrine regulatory network. The DβH gene was identified from the razor clam Sinonovacula constricta and referred to as ScDβH. The ScDβH gene is a copper type II ascorbate-dependent monooxygenase with a DOMON domain and two Cu2_monooxygen domains. ScDβH transcript expression was abundant in liver and hemolymph. During early development, ScDβH expression significantly increased at the umbo larval stage. Furthermore, the inhibitors and siRNA of DβH were screened. After challenge with DβH inhibitor, the larval metamorphosis and survival rates, and juvenile growth were obviously decreased. Under the siRNA stress, the larval metamorphosis and survival rates were also significantly decreased. Therefore, ScDβH may play an important regulating role in larval metamorphosis and juvenile growth.
多巴胺 β-羟化酶（DβH）在神经内分泌调节网络中儿茶酚胺（CA）的合成中起着关键作用。DβH 基因从中国蛤蜊（Sinonovacula constricta）中被鉴定出来，并被称为 ScDβH。ScDβH 基因是一种铜 II 型抗坏血酸依赖性单加氧酶，具有 DOMON 结构域和两个 Cu2_单加氧酶结构域。ScDβH 转录本在肝脏和血淋巴中表达丰富。在早期发育过程中，ScDβH 在耳状幼虫阶段的表达显著增加。此外，还筛选了 DβH 的抑制剂和 siRNA。用 DβH 抑制剂处理后，幼虫变态和存活率以及幼体生长明显下降。在 siRNA 应激下，幼虫变态和存活率也显著降低。因此，ScDβH 可能在幼虫变态和幼体生长中发挥重要的调节作用。
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