Heidari Hassan, Mammostafaei Chiman
Department of Chemistry, Azarbaijan Shahid Madani University, Tabriz, Iran.
Department of Chemistry, Azarbaijan Shahid Madani University, Tabriz, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Feb 15;247:119098. doi: 10.1016/j.saa.2020.119098. Epub 2020 Oct 24.
In this study, sensitive detection of lamotrigine in human plasma samples was realized at a low cost approach through ultrasound-assisted emulsification-microextraction based on using a hydrophobic deep eutectic solvent followed by back-extraction (USAEME-DES-BE) method. After extraction, detection and quantification of lamotrigine were done by spectrophotometry in the UV region. The hydrophobicity of the deep eutectic solvent not only eliminates the need of the third solvent as an emulsifying agent but also helps to retrieve lamotrigine from the DES by back-extraction to another aqueous phase. The back extraction process allowed the drug to be measured in the UV region. Central composite design in combination with a desirability function approach was applied for the optimization of the USAEME-DES-BE procedure. Essential factors in the method efficiency were discussed, such as back-extraction solution, time of back-extraction, the ratio of DES components, pH, the volume of DES, salt concentration, and sonication time. The method exhibited a wide dynamic linear range from 0.5 to 10 µg mL and a limit of detection of 0.15 μg mL. The established method was successfully applied to determine lamotrigine in human plasma samples with satisfactory relative recoveries.
在本研究中，基于使用疏水性深共熔溶剂并进行反萃取的超声辅助乳化微萃取（USAEME-DES-BE）方法，以低成本方式实现了对人血浆样本中拉莫三嗪的灵敏检测。萃取后，通过紫外区域的分光光度法对拉莫三嗪进行检测和定量。深共熔溶剂的疏水性不仅消除了使用第三种溶剂作为乳化剂的需求，还有助于通过反萃取将拉莫三嗪从深共熔溶剂中回收至另一水相。反萃取过程使得药物能够在紫外区域进行测定。采用中心复合设计结合期望函数法对USAEME-DES-BE程序进行优化。讨论了方法效率的关键因素，如反萃取溶液、反萃取时间、深共熔溶剂成分比例、pH值、深共熔溶剂体积、盐浓度和超声时间。该方法具有0.5至10 μg/mL的宽动态线性范围和0.15 μg/mL的检测限。所建立的方法成功应用于测定人血浆样本中的拉莫三嗪，相对回收率令人满意。
