Zohdijamil Zeynab, Reza Ahmadi Afshar Seyed Ahmad, Khazalpour Sadegh, Hashemi Mahdi
Faculty of Chemistry, Bu-Ali Sina University, Hamedan, Iran.
Anal Methods. 2021 Feb 21;13(7):946-954. doi: 10.1039/d0ay02197k. Epub 2021 Feb 2.
A new and simple deep eutectic solvent based ultrasound-assisted emulsification microextraction (DES-UAEME) procedure has been developed for preconcentration and voltammetric determination of aflatoxin B1 (AFB1) in cereal products. The method is based on the acetonitrile-based extraction of AFB1 from homogenized cereal samples followed by a DES-UAEME procedure for subsequent differential pulse voltammetry (DPV) determination in a microcell. A DES composed of choline chloride and urea (ChCl-Ur) was used as the extraction solvent and electrolyte for DPV detection. Various parameters affecting the extraction efficiency of AFB1 were evaluated and optimized. Under optimum conditions the calibration graph was linear in the range of 0.2-80.0 μg L (R = 0.9966) and the limit of detection (3S) was estimated to be 0.05 μg L. The intra-day and inter-day precision (RSD%) for determination of 5.0 μg L AFB1 were 3.4% and 3.9%, respectively. The proposed method was also successfully applied for preconcentration and determination of AFB1 in different cereal samples and good relative recoveries were obtained over a range of 94 to 104%.
已开发出一种基于新型简单的深共熔溶剂的超声辅助乳化微萃取（DES-UAEME）方法，用于谷物产品中黄曲霉毒素B1（AFB1）的预浓缩和伏安测定。该方法基于从均质谷物样品中用乙腈萃取AFB1，然后采用DES-UAEME方法在微池中进行后续差分脉冲伏安法（DPV）测定。由氯化胆碱和尿素（ChCl-Ur）组成的深共熔溶剂用作萃取溶剂和用于DPV检测的电解质。评估并优化了影响AFB1萃取效率的各种参数。在最佳条件下，校准曲线在0.2 - 80.0 μg L范围内呈线性（R = 0.9966），检测限（3S）估计为0.05 μg L。测定5.0 μg L AFB1的日内和日间精密度（RSD%）分别为3.4%和3.9%。所提出的方法也成功应用于不同谷物样品中AFB1的预浓缩和测定，在94%至104%的范围内获得了良好的相对回收率。
