Thomas D, Surdin-Kerjan Y
Laboratoire d'Enzymologie, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
J Biol Chem. 1987 Dec 5;262(34):16704-9.
Saccharomyces cerevisiae contains two genes, SAM1 and SAM2, encoding functional S-adenosylmethionine synthetases. The gene SAM1 was isolated by functional complementation of a double mutant of S. cerevisiae, and its identity was confirmed by gene disruption. The cloned gene was used to probe wild type chromosomal DNA, and two regions hybridizing with SAM1 were found, one of which is the SAM1 region. The DNA sequence of SAM1 is reported. The translation product shows a high homology with the one deduced from the sequence of the MetK gene encoding the SAM synthetase of Escherichia coli.
酿酒酵母含有两个基因，SAM1和SAM2，它们编码功能性S-腺苷甲硫氨酸合成酶。通过酿酒酵母双突变体的功能互补分离出基因SAM1，并通过基因破坏证实了其身份。克隆的基因用于探测野生型染色体DNA，发现了两个与SAM1杂交的区域，其中一个是SAM1区域。报道了SAM1的DNA序列。翻译产物与从编码大肠杆菌SAM合成酶的MetK基因序列推导的产物具有高度同源性。
