氯化钠浓度对氯化钠辅助厌氧发酵中微生物特性的影响：水解酶活性和微生物群落分布
Effect of NaCl Concentration on Microbiological Properties in NaCl Assistant Anaerobic Fermentation: Hydrolase Activity and Microbial Community Distribution.
作者信息
Pang Heliang, Xin Xiaodong, He Junguo, Cui Baihui, Guo Dabin, Liu Shiming, Yan Zhongsen, Liu Chong, Wang Xinyu, Nan Jun
机构信息
School of Environment, Harbin Institute of Technology, Harbin, China.
Department of Environmental Science and Engineering, Huaqiao University, Xiamen, China.
出版信息
Front Microbiol. 2020 Oct 9;11:589222. doi: 10.3389/fmicb.2020.589222. eCollection 2020.
Previous studies have demonstrated that sludge hydrolysis and short-chain fatty acids (SCFAs) production were improved through NaCl assistant anaerobic fermentation. However, the effect of NaCl concentrations on hydrolase activity and microbial community structure was rarely reported. In this study, it was found that α-glucosidase activity and some carbohydrate-degrading bacteria were inhibited in NaCl tests, owing to their vulnerability to high NaCl concentration. Correspondingly, the microbial community richness and diversity were reduced compared with the control test, while the evenness was not affected by NaCl concentration. By contrast, the protease activity was increased in the presence of NaCl and reached the highest activity at the NaCl concentration of 20 g/L. The protein-degrading and SCFAs-producing bacteria (e.g., and ) were enriched in the presence of NaCl, which were salt-tolerant.
先前的研究表明，通过NaCl辅助厌氧发酵可改善污泥水解和短链脂肪酸（SCFAs）的产生。然而，NaCl浓度对水解酶活性和微生物群落结构的影响鲜有报道。在本研究中发现，在NaCl试验中α-葡萄糖苷酶活性和一些碳水化合物降解细菌受到抑制，这是由于它们对高NaCl浓度较为敏感。相应地，与对照试验相比，微生物群落的丰富度和多样性降低，而均匀度不受NaCl浓度影响。相比之下，在有NaCl存在的情况下蛋白酶活性增加，并在NaCl浓度为20 g/L时达到最高活性。在有NaCl存在的情况下，蛋白质降解和SCFAs产生细菌（如 和 ）得以富集，这些细菌具有耐盐性。
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