Wang Jie, Liu Xingguo, He Jinling, Cheng Guofeng, Xu Junli, Lu Ming, Shangguan Yuyi, Zhang Ai
Fishery Machinery and Instrument Research Institute of Chinese Academy of Fishery Sciences, Shanghai, China.
Key Laboratory of Aquaculture Facilities Engineering, Ministry of Agriculture and Rural Affairs, Shanghai, China.
Front Microbiol. 2022 Jul 22;13:963260. doi: 10.3389/fmicb.2022.963260. eCollection 2022.
The mechanism of improving the anaerobic fermentation performance of waste active sludge by using dielectric barrier discharge (DBD) plasma pretreatment technology was investigated. The maximum accumulation of short-chain fatty acids (SCFAs) was observed on the 7th day of anaerobic fermentation when the DBD power was 76.50 W, which was 1726.70 mg COD/L, 1.50 times of the control group. The ratio of acetic acid in DBD group was 9.30% higher than that in the control. Further mechanism research indicated that DBD pretreatment can destroy the structure of extracellular polymer substances and release organic substances such as protein and polysaccharide. The dissolved organic matter analysis indicated that the DBD technique could increase the release of biodegradable organics (eg., tyrosine proteins, soluble microbial by-products), thus accelerate the biotransformation of organic substance. Bacterial community structure analysis showed that the increase in the abundance of Firmicutes and Bacteroidetes and the decrease in the abundance of Proteobacteria in DBD group were beneficial to the accumulation of SCFAs. Besides, further archaeal analysis indicated that the decrease of sp. and . abundance in the DBD group facilitate acetic acid accumulation. This study demonstrated that the DBD technique can be used as an effective and potential pretreatment method to improve sludge anaerobic fermentation performance.
研究了利用介质阻挡放电（DBD）等离子体预处理技术提高废弃活性污泥厌氧发酵性能的机制。当DBD功率为76.50W时，在厌氧发酵第7天观察到短链脂肪酸（SCFAs）的最大积累量，为1726.70mg COD/L，是对照组的1.50倍。DBD组中乙酸的比例比对照组高9.30%。进一步的机制研究表明，DBD预处理可以破坏细胞外聚合物的结构，并释放蛋白质和多糖等有机物。溶解性有机物分析表明，DBD技术可以增加可生物降解有机物（如酪氨酸蛋白、可溶性微生物副产物）的释放，从而加速有机物的生物转化。细菌群落结构分析表明，DBD组中厚壁菌门和拟杆菌门丰度的增加以及变形菌门丰度的降低有利于SCFAs的积累。此外，进一步的古菌分析表明，DBD组中sp.和.丰度的降低促进了乙酸的积累。本研究表明，DBD技术可作为一种有效且有潜力的预处理方法来提高污泥厌氧发酵性能。
