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从卡塔尔土壤中分离出的菌株,能合成对病媒昆虫具有高活性的δ-内毒素。

strains isolated from Qatari soil, synthesizing δ-endotoxins highly active against the disease vector insect .

作者信息

Nair Kavita, Al-Thani Roda, Ginibre Carole, Chandre Fabrice, Alsafran Mohammed, Jaoua Samir

机构信息

Department of Biological & Environmental Sciences, College of Arts & Sciences, Qatar University, Doha, P.O. Box 2713, Qatar.

IRD-MIVEGEC (UM-CNRS 5290-IRD 224), WHO Collaborating Center, Montpellier Cedex 5, France.

出版信息

Heliyon. 2020 Oct 23;6(10):e05003. doi: 10.1016/j.heliyon.2020.e05003. eCollection 2020 Oct.

Abstract

is a Gram-positive soil bacterium that has been recognized as an effective bioinsecticide active against plant, animal and human pathogenic and disease vector insects. During its sporulation phase, produces crystals consisting of δ-endotoxins, which upon ingestion kill specifically insect larvae. subsp. () is very active against dipteran insects. based bioinsecticides are considered as a sustainable solution to control the Dipteran insects responsible of plant, animal and human diseases. In this study, strains isolated from Qatar soil were analyzed for their insecticidal activities against the dipteran insect () and for their δ-endotoxins yields per cell. Among the local strains, four exceptional strains producing spherical crystals, were found to be more insecticidal than the reference strain H14. When tested for their δ-endotoxin yield, the QBT217 strain, producing typical spherical crystals and having the best insecticidal activity, was recognized as the best candidate strain for potential bioinsecticide production and biological control of dipteran insects, particularly the disease vector insect .

摘要

是一种革兰氏阳性土壤细菌,已被公认为是一种有效的生物杀虫剂,对植物、动物和人类的致病昆虫及病媒昆虫具有活性。在其芽孢形成阶段,会产生由δ-内毒素组成的晶体,这些晶体在被昆虫摄入后会特异性地杀死昆虫幼虫。亚种()对双翅目昆虫非常有效。基于的生物杀虫剂被认为是控制导致植物、动物和人类疾病的双翅目昆虫的可持续解决方案。在本研究中,对从卡塔尔土壤中分离出的菌株进行了分析,检测它们对双翅目昆虫()的杀虫活性以及每个细胞的δ-内毒素产量。在当地的菌株中,发现有四个产生球形晶体的特殊菌株比参考菌株H14更具杀虫活性。在检测它们的δ-内毒素产量时,产生典型球形晶体且具有最佳杀虫活性的QBT217菌株被认为是潜在生物杀虫剂生产和双翅目昆虫生物防治的最佳候选菌株,特别是对病媒昆虫。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/297f/7610223/0fda0c0240c0/gr3.jpg

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