使用SIB和API试剂盒鉴定不同来源的肠杆菌并进行生物分型。

Using SIB and API kits to identify and biotype enterobacteria of different origin.

作者信息

Kulinich L I, Fish N G, Mazitova O P, Dratvin S A, Birger M O

机构信息

Laboratory of Immunochemistry, All-Union Research Institute of Protein Substances Biosynthesis, Moskva, USSR.

出版信息

J Hyg Epidemiol Microbiol Immunol. 1987;31(3):287-92.

PMID:3316379

Abstract

A collection of 26 Enterobacteriaceae reference strains provided by Reference Centres in Moscow (USSR) and Copenhagen (Denmark) as well as a collection of 660 freshly isolated cultures of Gram-negative bacteria of different origin were investigated using SIB indicator systems manufactured at the Gorky Institute of Epidemiology and Microbiology (USSR) and API-20E, Rapid-20E and API-10S kits (API, France) with the aim of species determination. In analyzing freshly isolated cultures, API-20E, API-10S and SIB-B kits proved to be of approximately equal efficiency, whereas the Rapid-20E system enabled species identification in no more than 78% of the tested cultures. In a model biotyping of 284 E. coli cultures of different origin, SIB-B and API-20E kits in combination with the Analytical Profile Index enabled sufficiently rapid and standard identification of Enterobacteriaceae biovars.

摘要

对由莫斯科（苏联）和哥本哈根（丹麦）的参考中心提供的26株肠杆菌科参考菌株，以及660株不同来源的新分离革兰氏阴性细菌培养物进行了研究，使用了苏联高尔基流行病学和微生物学研究所生产的SIB指示系统以及API - 20E、Rapid - 20E和API - 10S试剂盒（法国API公司），目的是进行菌种鉴定。在分析新分离的培养物时，API - 20E、API - 10S和SIB - B试剂盒的效率大致相当，而Rapid - 20E系统在不超过78%的测试培养物中能够进行菌种鉴定。在对284株不同来源的大肠杆菌培养物进行的模型生物分型中，SIB - B和API - 20E试剂盒与分析谱指数相结合，能够充分快速且标准地鉴定肠杆菌科生物变种。