Lopez Augustin, Fayolle Dimitri, Fiore Michele, Strazewski Peter
Université de Lyon, Université Claude Bernard Lyon 1, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, Bâtiment Edgar Lederer, 1 Rue Victor Grignard, 69622 Villeurbanne Cedex, France.
iScience. 2020 Oct 15;23(11):101677. doi: 10.1016/j.isci.2020.101677. eCollection 2020 Nov 20.
The reproduction of the shape of giant vesicles usually results in the increase of their "population" size. This may be achieved on giant vesicles by appropriately supplying "mother" vesicles with membranogenic amphiphiles. The next "generation" of "daughter" vesicles obtained from this "feeding" is inherently difficult to distinguish from the original mothers. Here we report on a method for the consecutive feeding with different fatty acids that each provoke membrane growth and detachment of daughter vesicles from glass microsphere-supported phospholipidic mother vesicles. We discovered that a saturated fatty acid was carried over to the next generation of mothers better than two unsaturated congeners. This has an important bearing on the growth and replication of primitive compartments at the early stages of life. Microsphere-supported vesicles are also a precise analytical tool.
巨型囊泡形状的复制通常会导致其“群体”规模的增加。这可以通过向巨型囊泡适当供应成膜两亲物来实现。通过这种“投喂”获得的下一代“子代”囊泡本质上很难与原来的母囊泡区分开来。在此，我们报告一种用不同脂肪酸连续投喂的方法，每种脂肪酸都会引发膜生长以及子代囊泡从玻璃微球支撑的磷脂母囊泡上脱离。我们发现，一种饱和脂肪酸比两种不饱和同系物更能传递到下一代母囊泡中。这对于生命早期原始区室的生长和复制具有重要意义。微球支撑的囊泡也是一种精确的分析工具。
