Thomas N, Bennett R, Jones C N
Amersham International, Whitchurch, Cardiff, Wales, U.K.
J Immunol Methods. 1987 Nov 23;104(1-2):201-7. doi: 10.1016/0022-1759(87)90505-9.
A method is described which allows rapid and quantitative comparison of immunocytochemical staining procedures. Cells grown and fixed in microtitre plates are probed with increasing dilutions of primary antibody and then stained using the procedures under test; the resulting staining intensities are determined using a microtitre plate reader. The microtitre immunocytochemistry assay (MIA) has been used to compare the sensitivities of enhancement procedures based on immunoperoxidase and immunogold staining. Silver enhancement of DAB staining was found to be the most sensitive technique giving up to 200 fold amplification of the peroxidase staining.
本文描述了一种可对免疫细胞化学染色程序进行快速定量比较的方法。在微量滴定板中生长并固定的细胞，用浓度递增的一抗进行检测，然后使用受试程序进行染色；使用微量滴定板读数器测定所得的染色强度。微量滴定免疫细胞化学分析（MIA）已用于比较基于免疫过氧化物酶和免疫金染色的增强程序的灵敏度。发现DAB染色的银增强是最灵敏的技术，可使过氧化物酶染色放大多达200倍。
