Cuo Nan, Rui G U, Lin F U, Ke-Ru Wang, Rong Ding, Cai-Hong Zhang
College of Ethnomedicine, Chengdu University of Traditional Chinese Medicine Chengdu 611137, China.
College of Pharmacy, Chengdu University of Traditional Chinese Medicine Chengdu 611137, China Sichuan New Green Pharmaceutical Chengdu 611137, China.
Zhongguo Zhong Yao Za Zhi. 2020 Sep;45(18):4423-4428. doi: 10.19540/j.cnki.cjcmm.20200603.202.
In this study, the content of iridoids and flavonoids in Tibetan medicine "Lanhua Longdan" was determined simul-taneously by quantitative analysis of multi-components by single marker(QAMS), which was used to verify the feasibility and applicability of the method in the application of Lanhua Longdan quality evaluation. Using HPLC with two typical elements gentiopicroside and isoorientin as the internal reference, the relative correction factor(RCF) between the mand loganin acid, swertiamarin, sweroside, isoscoparin-2″-β-D-glucopyranoside and isoscoparin was determined and then used, to calculate the content of several other components to achieve QAMS. At the same time, the external standard method(ESM) was used to determine the contents of these 7 components in the medicinal materials, and the differences were compared to verify the accuracy and feasibility of QAMS. The results showed that the RCF repeatability is good. There were no significant differences in the determination results of the contents of 12 batches of 4 varieties of Tibetan medicine Lanhua Longdan obtained by QAMS and ESM. Therefore, the QAMS can be used for the quality evaluation of Lanhua Longdan, and provides a reference for the quality evaluation of multi-index components of Lanhua Longdan. The results showed that the content of iridoid and flavonoid in the dried product should not be less than 0.6% and 0.8% respectively.
本研究采用一测多评法（QAMS）同时测定藏药“兰花龙胆”中环烯醚萜类和黄酮类成分的含量，以验证该方法在兰花龙胆质量评价应用中的可行性和适用性。以高效液相色谱法，选用龙胆苦苷和异荭草素这两种典型成分作为内参物，测定马钱子酸、獐牙菜苦苷、獐牙菜苷、异荭草苷 -2″-β -D-葡萄糖苷与异荭草素之间的相对校正因子（RCF），进而计算其他几种成分的含量，实现一测多评。同时，采用外标法（ESM）测定药材中这7种成分的含量，并比较两者差异，以验证一测多评法的准确性和可行性。结果表明，相对校正因子重复性良好。一测多评法与外标法测定4个品种12批次藏药兰花龙胆的含量结果无显著差异。因此，一测多评法可用于兰花龙胆的质量评价，为兰花龙胆多指标成分的质量评价提供参考。结果表明，干燥品中环烯醚萜类和黄酮类成分的含量分别不得少于0.6%和0.8%。
