Zhao Wen-Wen, Wu Zhi-Min, Wu Xia, Zhao Hai-Yu, Chen Xiao-Qing
School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
Beijing Key Lab of Traditional Chinese Medicine Collateral Disease Theory Research,Beijing 100069, China.
Zhongguo Zhong Yao Za Zhi. 2016 Oct;41(20):3792-3797. doi: 10.4268/cjcmm20162014.
This study is to determine five naphthaquinones (acetylshikonin, β-acetoxyisovalerylalkannin, isobutylshikonin, β,β'-dimethylacrylalkannin,α-methyl-n-butylshikonin） by quantitative analysis of multi-components with a single marker (QAMS). β,β'-Dimethylacrylalkannin was selected as the internal reference substance, and the relative correlation factors (RCFs) of acetylshikonin, β-acetoxyisovalerylalkannin, isobutylshikonin and α-methyl-n-butylshikonin were calculated. Then the ruggedness of relative correction factors was tested on different instruments and columns. Meanwhile, 16 batches of Arnebia euchroma were analyzed by external standard method (ESM) and QAMS, respectively. The peaks were identifited by LC-MS. The ruggedness of relative correction factors was good. And the analytical results calculated by ESM and QAMS showed no difference. The quantitative method established was feasible and suitable for the quality evaluation of A. euchroma.
本研究旨在采用一测多评法（QAMS）测定五种萘醌类成分（乙酰紫草素、β-乙酰氧基异戊酰紫草素、异丁基紫草素、β，β'-二甲基丙烯酰紫草素、α-甲基-n-丁基紫草素）。选取β，β'-二甲基丙烯酰紫草素作为内参物，计算乙酰紫草素、β-乙酰氧基异戊酰紫草素、异丁基紫草素和α-甲基-n-丁基紫草素的相对校正因子（RCFs）。然后在不同仪器和色谱柱上考察相对校正因子的耐用性。同时，分别采用外标法（ESM）和QAMS对16批新疆紫草进行分析。通过液相色谱-质谱联用（LC-MS）对色谱峰进行鉴定。相对校正因子的耐用性良好。并且用ESM和QAMS计算得到的分析结果无差异。所建立的定量方法可行，适用于新疆紫草的质量评价。
