Gang Fan, Qi L I, Xin-Mei X U, Huan D U, Tong X U, Xian-Rong Lai, Lei-Lei D U
College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine Chengdu 611137, China.
College of Pharmacy, Chengdu University of Traditional Chinese Medicine Chengdu 611137, China.
Zhongguo Zhong Yao Za Zhi. 2020 Oct;45(19):4677-4685. doi: 10.19540/j.cnki.cjcmm.20200620.201.
To evaluate the quality differences of four mainstream species of Berberidis Cortex,~1H-NMR metabolomics was applied to detect its primary and secondary metabolites, and the partial least squares discriminant analysis and analysis of variance were integrated to screen differential metabolites between species. Furthermore, diabetic rat model was established by high fat diet and streptozotocin to assess differences in antidiabetic activities among the four species. Sixteen compounds were simultaneously detected and identified, including alkaloids, organic acids, carbohydrates and amino acids. Interspecific difference markers were revealed as magnoflorine, jateorhi-zine, bufotenidine and saccharose for the first time. Berberis vernae and B. kansuensis presented superior activities on reducing blood glucose level, improving insulin resistance, increasing insulin sensibility and anti-inflammation. B. dictyophylla showed moderate antidiabe-tic effect, while B. diaphana rendered inferior antidiabetic capacity. Based on the contents of four differential markers and the results of antidiabetic activity evaluation, the quality of four Berberidis Cortex species was ranked as B.vernae≈B.kansuensis>B. dictyophylla>B. diaphana. These results provided references for species collation, quality standard establishment and exploitation of Berberidis Cortex. The antidiabetic activities of B. vernae and B. kansuensis as well as their mechanisms of action merit further study in the future.
为评价四种主流黄柏药材的质量差异，采用氢核磁共振代谢组学技术检测其初级和次级代谢产物，并结合偏最小二乘判别分析和方差分析筛选不同种间的差异代谢产物。此外，通过高脂饮食和链脲佐菌素建立糖尿病大鼠模型，以评估这四种黄柏药材在抗糖尿病活性方面的差异。同时检测并鉴定出16种化合物，包括生物碱、有机酸、碳水化合物和氨基酸。首次揭示了种间差异标志物为木兰碱、药根碱、蟾蜍特尼定和蔗糖。秦岭黄柏和甘肃黄柏在降低血糖水平、改善胰岛素抵抗、提高胰岛素敏感性和抗炎方面表现出较强的活性。大叶黄柏表现出中等程度的抗糖尿病作用，而峨眉黄柏的抗糖尿病能力较弱。基于四种差异标志物含量及抗糖尿病活性评价结果，四种黄柏药材的质量排序为：秦岭黄柏≈甘肃黄柏＞大叶黄柏＞峨眉黄柏。这些结果为黄柏药材的品种整理、质量标准制定及开发利用提供了参考依据。秦岭黄柏和甘肃黄柏的抗糖尿病活性及其作用机制值得未来进一步深入研究。
