[何首乌及制何首乌水提取物对MCD诱导的小鼠非酒精性脂肪性肝炎的改善作用研究]
[Study on improvement provided by water extracts of Polygoni Multiflori Radix and Polygoni Multiflori Radix Praeparata on non-alcoholic steatohepatitis in mice induced by MCD].
作者信息
Fei-Fei H U, Zhan-Xia Hao, Shao-Bo Zhang, Yu-Chen Sheng, Li-Li J I
机构信息
the MOE Key Laboratory for Standardization of Chinese Medicines, the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Shanghai Key Laboratory of Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine Shanghai 201203, China Center for Drug Safety Evaluation and Research, Innovation Research Institute of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine Shanghai 201203, China.
the MOE Key Laboratory for Standardization of Chinese Medicines, the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Shanghai Key Laboratory of Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine Shanghai 201203, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2020 Oct;45(19):4732-4739. doi: 10.19540/j.cnki.cjcmm.20200610.401.
This study aims to observe the improvement of non-alcoholic steatohepatitis(NASH) after using water extracts of Polygoni Multiflori Radix and Polygoni Multiflori Radix Praeparata and explore their preliminary mechanism. Mice were fed with methionine-choline-deficent diet(MCD) for 6 weeks for modeling, and mice were orally given with 50, 100, 200 mg·kg(-1) of Polygoni Multiflori Radix water extract(PMRWE) or Polygoni Multiflori Radix Praeparata water extract(PMRPWE) at the last 4 weeks. During the whole experimental procedure, the body weight changes of the mice were monitored and recorded. Serum alanine aminotransferase(ALT) and aspartate aminotransferase(AST) activities were detected; liver histopathological evaluation and NAFLD activity score(NAS) calculation were conducted, and the levels of reactive oxygen species(ROS) in liver tissues were analyzed. The contents of triglyceride(TG) and non-esterified fatty acids(NEFA) in liver tissues were detected, and oil red O staining of the liver tissues was conducted and observed. Quantitative polymerase chain reaction(qPCR) was used to detect hepatic mRNA expression of β-oxidation-related genes in mice. The results showed that PMRWE(100, 200 mg·kg(-1)) and PMRPWE(50, 100, 200 mg·kg(-1)) alleviated liver damage in MCD-induced NASH in mice. PMRWE(100, 200 mg·kg(-1)) and PMRPWE(50, 100, 200 mg·kg~(-1)) reduced hepatic li-pid accumulation in mice with NASH. Different doses of PMRPWE inversed the decreased hepatic mRNA expression of β-oxidation-related genes in mice with NASH. This study indicated that PMRPWE and PMRWE could ameliorate MCD-induced NASH in mice by promoting fatty acid β oxidation, reducing liver lipid accumulation, and alleviating liver damage. Moreover, the protective effect of PMRPWE against MCD-induced NASH was better than PMRWE.
本研究旨在观察何首乌及制何首乌水提取物对非酒精性脂肪性肝炎（NASH）的改善作用，并探讨其初步机制。将小鼠喂以蛋氨酸-胆碱缺乏饮食（MCD）6周以建立模型，在最后4周给小鼠口服50、100、200 mg·kg⁻¹的何首乌水提取物（PMRWE）或制何首乌水提取物（PMRPWE）。在整个实验过程中，监测并记录小鼠的体重变化。检测血清丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）活性；进行肝脏组织病理学评估并计算非酒精性脂肪性肝病活动度评分（NAS），分析肝组织中活性氧（ROS）水平。检测肝组织中甘油三酯（TG）和非酯化脂肪酸（NEFA）含量，并对肝组织进行油红O染色观察。采用定量聚合酶链反应（qPCR）检测小鼠肝脏中β-氧化相关基因的mRNA表达。结果表明，PMRWE（100、200 mg·kg⁻¹）和PMRPWE（50、100、200 mg·kg⁻¹）可减轻MCD诱导的小鼠NASH肝损伤。PMRWE（100、200 mg·kg⁻¹）和PMRPWE（50、100、200 mg·kg⁻¹）可减少NASH小鼠肝脏脂质蓄积。不同剂量的PMRPWE可逆转NASH小鼠肝脏中β-氧化相关基因mRNA表达的降低。本研究表明，PMRPWE和PMRWE可通过促进脂肪酸β氧化、减少肝脏脂质蓄积及减轻肝损伤来改善MCD诱导的小鼠NASH。此外，PMRPWE对MCD诱导的NASH的保护作用优于PMRWE。
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