Zhang Chao, Zhang Rui-Chen, Sun Zhen-Xiao
Zhong Yao Cai. 2013 Sep;36(9):1416-9.
To compare the hepatoxicity of Polygoni Multiflori Radix and Polygoni Multiflori Rodix Praeparata in rats.
Water extracts of Polygoni Multiflori Radix and Polygoni Multiflori Rodix Praeparata were prepared by concentional decocting method (named as RPWE and PPWE respectively). Both in 30 days and 60 days experiments, SD rats were randomly divided into 5 groups: normal control group, RPWE high dosage group (40 mg/kg x d) and PPWE low dosage group (20 mg/kg x d), PPWE high dosage group (40 mg/kg x d) and PPEW low dosage group (20 mg/kg x d). After daily administration,the general conditions of rats were observed. At the end of the experiments, the levels of ALT and AST in plasma were detected. The liver coefficient was calculated and HE staining was used to observe the changes of liver tissue sections.
Both in 30 days and 60 days experiments, the treatment groups rats had hypokinesia and dry fur when compared with control groups, and in a dose-dependent manner. The liver coefficients, levels of ALT and AST of plasma weren't changed significantly when conpared with control groups. The liver had varying degrees of steatosis and inflammation cell infiltration in the treatment groups rats in 60 days experiment.
Oral administration of Polygoni Multiflori Radix and Polygoni Multiflori Rodix Praeparata for 60 days have hepatoxic in rats.
比较生何首乌与制何首乌对大鼠的肝毒性。
采用常规煎煮法制备生何首乌水提取物（分别命名为RPWE）和制何首乌水提取物（PPWE）。在30天和60天的实验中，将SD大鼠随机分为5组：正常对照组、RPWE高剂量组（40mg/kg·d）、PPWE低剂量组（20mg/kg·d）、PPWE高剂量组（40mg/kg·d）和PPEW低剂量组（20mg/kg·d）。每日给药后，观察大鼠的一般情况。实验结束时，检测血浆中ALT和AST水平。计算肝脏系数，并用HE染色观察肝组织切片的变化。
在30天和60天的实验中，与对照组相比，各治疗组大鼠均出现运动减少、皮毛干燥，且呈剂量依赖性。与对照组相比，各治疗组大鼠的肝脏系数、血浆ALT和AST水平均无明显变化。在60天的实验中，各治疗组大鼠的肝脏均有不同程度的脂肪变性和炎症细胞浸润。
生何首乌与制何首乌连续灌胃60天对大鼠有肝毒性。
