Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, Liaoning, China.
Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng, Liaoning, China.
Genomics. 2021 Jan;113(1 Pt 2):919-926. doi: 10.1016/j.ygeno.2020.10.025. Epub 2020 Oct 22.
How lipids influence post-harvest softening in pears is not well understood. LC-MS/MS (Liquid chromatography-tandem mass spectrometry) and RNA-Seq analyses of 'Zaoshu Shanli' (ZSSL) pears were conducted during post-harvest storage. This approach enabled the identification of 98 different metabolites that upregulated and 95 that downregulated at 18 days post-harvest in ZSSL fruits to day 0. Metabolites were significantly enriched in KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways including glycerophospholipid metabolism and glycosylphosphatidylinositol (GPI)-anchor biosynthesis. When comparing fruits from day 18 to those from day 0 post-harvest, RNA-seq analyses further highlighted 6496 differentially expressed genes (DEGs) in ZSSL fruits that were significantly enriched in KEGG pathways including glycerophospholipid metabolism and fatty acid degradation. Overall, these results suggested that glycerophospholipid metabolism is closely related to the post-harvest softening of pears. Further research will be essential in order to fully explore the functional implications of and mechanistic basis for these findings.
脂质如何影响梨的采后软化尚不清楚。对‘早酥山梨’（ZSSL）梨进行了 LC-MS/MS（液相色谱-串联质谱）和 RNA-Seq 分析，以研究其在采后贮藏过程中的变化。这种方法可以鉴定出 98 种在 ZSSL 果实采后 18 天和 0 天之间上调的不同代谢物和 95 种下调的代谢物。代谢物在 KEGG（京都基因和基因组百科全书）途径中显著富集，包括甘油磷脂代谢和糖基磷脂酰肌醇（GPI）锚生物合成。当比较采后 18 天和 0 天的果实时，RNA-seq 分析进一步突出了 ZSSL 果实中 6496 个差异表达基因（DEGs），这些基因在甘油磷脂代谢和脂肪酸降解等 KEGG 途径中显著富集。总的来说，这些结果表明甘油磷脂代谢与梨的采后软化密切相关。为了充分探索这些发现的功能意义和机制基础，进一步的研究将是必不可少的。
